Maas J W, Hattox S E, Landis D H, Roth R H
Eur J Pharmacol. 1977 Dec 1;46(3):221-8. doi: 10.1016/0014-2999(77)90337-5.
A direct method for measuring the rate of production of neurotransmitter metabolites by the brain of awake monkeys is described. The method utilizes a coupling of a measure of cerebral blood flow with the determination of the difference in concentration of the metabolite under study in arterial and internal jugular bulb blood. A consistent veno-arterial difference for 3-methoxy-4-hydroxyphenethylenglycol (MHPG) has been found. The concentration of MHPG in blood obtained from the right and left venous outflows from brain were not significantly different indicating that blood from either the right or left internal jugular bulb may be used with this method. The rate of production of MHPG by the brain of thw awake monkey is estimated to be 24.1 ng/100 g brain/min. The rate of MHPG production by brain is increased by the administration of piperoxan and decreased by clonidine. Using the experimentally determined rate of production of MHPG by brain and extrapolating to the human it is suggested that a substantial fraction of the total body production of MHPG in man occurs in brain.
本文描述了一种直接测量清醒猴子大脑中神经递质代谢产物生成速率的方法。该方法将脑血流量测量与所研究代谢产物在动脉血和颈内静脉球部血中浓度差异的测定相结合。已发现3-甲氧基-4-羟基苯乙二醇（MHPG）存在一致的静脉-动脉差异。从大脑右侧和左侧静脉流出的血液中MHPG的浓度无显著差异，这表明使用该方法时，右侧或左侧颈内静脉球部的血液均可使用。清醒猴子大脑中MHPG的生成速率估计为24.1纳克/100克脑/分钟。给予哌罗克生可使大脑中MHPG的生成速率增加，而可乐定则使其降低。利用实验确定的大脑中MHPG的生成速率并外推至人类，提示人类全身MHPG生成总量的很大一部分发生在大脑中。
