Moore D L, Heyworth B, Brown J
Immunology. 1977 Dec;33(6):777-85.
Phytohaemagglutinin (PHA) induced lymphocyte transformation in whole blood and in purified lymphocyte cultures was investigated in Gambian children with acute Plasmodium falciparum malaria or with acute protein-energy malnutrition (PEM). Responses of purified lymphocytes cultured in the absence of autologous plasma were normal, with one exception. Autologous plasma depressed the response of purified lymphocytes to a low dose of PHA in several malaria and PEM patients. In whole blood cultures of 1 day and of 3 day duration, responses of several children with malaria or PEM were less than those of control children. Responses were not related to absolute lymphocyte counts. In 3 day, but not 1 day, cultures from control and malarious children, responses were inversely proportional to neutrophil counts. Cultures of whole blood and of purified lymphocytes in autologous plasma gave comparable results in 58 of 70 patients.
对患有急性恶性疟原虫疟疾或急性蛋白质 - 能量营养不良（PEM）的冈比亚儿童，研究了植物血凝素（PHA）在全血和纯化淋巴细胞培养物中诱导的淋巴细胞转化情况。在没有自体血浆的情况下培养的纯化淋巴细胞的反应是正常的，但有一个例外。在一些疟疾和PEM患者中，自体血浆抑制了纯化淋巴细胞对低剂量PHA的反应。在持续1天和3天的全血培养中，几名患有疟疾或PEM的儿童的反应低于对照儿童。反应与绝对淋巴细胞计数无关。在对照儿童和患疟疾儿童的3天培养中（而非1天培养），反应与中性粒细胞计数成反比。在70名患者中的58名患者中，全血和在自体血浆中纯化淋巴细胞的培养得到了可比的结果。
