Keller R
Clin Exp Immunol. 1973 Jan;13(1):139-47.
Incubation of sensitized peritoneal or pleural cells, or isolated mast cells, taken from rats previously infected with the nematode, , but not of cells taken from parasite-free controls, with concanavalin A induces the release of histamine. The release mechanism triggered by Con A is virtually completed within 60 sec, depends on temperature, but not on the presence of complement, and is inhibited by sympathomimetic amines. It therefore shows striking similarities to the anaphylactic reaction induced by the specific allergen. The mechanism by which histamine release is triggered by Con A is discussed, considering the observations that trypsinized Con A is no longer able to induce histamine release, that the presence of high concentrations of trypsinized Con A inhibits the release of histamine by subsequently added Con A or allergen, and that certain carbohydrates inhibit the release of histamine induced by both Con A and allergen. Although some of the data suggest a similarity in the structure of the antigenic determinant in the Fab fragment of IgE and the receptor site for Con A, it seems more likely that Con A is reacting mainly by cross-linking of Fc regions of the immunoglobulin molecule.
用伴刀豆球蛋白A孵育先前感染过线虫的大鼠的致敏腹膜或胸膜细胞、或分离的肥大细胞时，会诱导组胺释放，但用来自未感染寄生虫的对照大鼠的细胞孵育时则不会。伴刀豆球蛋白A触发的释放机制在60秒内基本完成，取决于温度，但不取决于补体的存在，并且会被拟交感神经胺抑制。因此，它与特定过敏原诱导的过敏反应有显著相似之处。考虑到以下观察结果，讨论了伴刀豆球蛋白A触发组胺释放的机制：胰蛋白酶处理过的伴刀豆球蛋白A不再能够诱导组胺释放；高浓度的胰蛋白酶处理过的伴刀豆球蛋白A的存在会抑制随后添加的伴刀豆球蛋白A或过敏原诱导的组胺释放；某些碳水化合物会抑制伴刀豆球蛋白A和过敏原诱导的组胺释放。尽管一些数据表明IgE的Fab片段中的抗原决定簇结构与伴刀豆球蛋白A的受体位点有相似之处，但伴刀豆球蛋白A似乎更可能主要通过免疫球蛋白分子Fc区域的交联起反应。
