在AKR小鼠肾脏洗脱液中检测针对鼠白血病病毒RNA依赖性DNA聚合酶的天然抗体。
Detection of naturally occurring antibodies to RNA-dependent DNA polymerase of murine leukemia virus in kidney eluates of AKR mice.
作者信息
Hollis V W, Aoki T, Barrera O, Oldstone M B, Dixon F J
出版信息
J Virol. 1974 Feb;13(2):448-54. doi: 10.1128/JVI.13.2.448-454.1974.
Abstract
Specific antibodies to the RNA-dependent DNA polymerase (reverse transcriptase) of murine type C viruses have been isolated from the renal glomeruli of both leukemic and nonleukemic AKR mice where they presumably had been deposited as immune complexes. The antibodies were shown to have sedimentation coefficients of 26S to 28S and 5S to 7S on sucrose rate zonal centrifugation. Inactivation with monospecific antisera to various mouse immunoglobulins identified antibodies as being in both immunoglobulin (IGM) and IgG classes. In addition, these antibodies only reacted with the reverse transcriptase from murine and feline type C viruses, but not the polymerase from avian myeloblastosis virus (AMV). Our results provide additional evidence for the lack of immunological tolerance and demonstrate the presence of another immune complex system in AKR kidneys.
摘要
从小鼠白血病病毒和非白血病AKR小鼠的肾小球中分离出了针对鼠C型病毒RNA依赖性DNA聚合酶（逆转录酶）的特异性抗体，这些抗体可能以免疫复合物的形式沉积在肾小球中。在蔗糖速率区带离心中，这些抗体的沉降系数显示为26S至28S和5S至7S。用针对各种小鼠免疫球蛋白的单特异性抗血清进行灭活鉴定，发现抗体存在于免疫球蛋白（IGM）和IgG类别中。此外，这些抗体仅与鼠和猫C型病毒的逆转录酶反应，而不与禽成髓细胞瘤病毒（AMV）的聚合酶反应。我们的结果为缺乏免疫耐受性提供了更多证据，并证明了AKR肾脏中存在另一种免疫复合物系统。
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