Rocklin R E, Pence H, Kaplan H, Evans R
J Clin Invest. 1974 Mar;53(3):735-44. doi: 10.1172/JCI107612.
The in vivo and in vitro responses to ragweed antigen E were evaluated in 28 untreated atopic patients with ragweed hayfever. The methods employed included direct skin testing, measurement of total serum IgE, measurement of specific IgE anti-ragweed antibodies, leukocyte histamine release, lymphocyte transformation, and release of lymphocyte mediators (migration inhibitory factor and mitogenic factor). The patients could be divided into sensitive and insensitive groups on the basis of their in vitro reactivity to antigen E. 20 patients in the sensitive group had statistically higher levels of total serum IgE, higher levels of specific IgE anti-ragweed antibodies, and greater leukocyte sensitivity as measured by antigen-induced histamine release than did eight patients in the insensitive group. Lymphocytes from sensitive patients produced greater amounts of migration inhibitory factor and mitogenic factor when challenged by antigen E than did lymphocytes from insensitive patients. A possible role for the lymphocyte in this allergic disease is discussed. The results of this study indicate that the immune response to ragweed antigen is complex and involves components of both immediate and delayed hypersensitivity.
对28名未经治疗的豚草花粉热特应性患者进行了体内和体外对豚草抗原E的反应评估。所采用的方法包括直接皮肤试验、总血清IgE测量、特异性IgE抗豚草抗体测量、白细胞组胺释放、淋巴细胞转化以及淋巴细胞介质（迁移抑制因子和促有丝分裂因子）释放。根据患者对抗原E的体外反应性，可将其分为敏感组和不敏感组。敏感组的20名患者在统计学上总血清IgE水平更高、特异性IgE抗豚草抗体水平更高，并且通过抗原诱导的组胺释放测量的白细胞敏感性比不敏感组的8名患者更高。当受到抗原E攻击时，敏感患者的淋巴细胞比不敏感患者的淋巴细胞产生更多的迁移抑制因子和促有丝分裂因子。讨论了淋巴细胞在这种过敏性疾病中的可能作用。本研究结果表明，对豚草抗原的免疫反应是复杂的，涉及速发型和迟发型超敏反应的成分。
