Shay L K, Vary J C
Biochim Biophys Acta. 1978 Jan 18;538(2):284-92. doi: 10.1016/0304-4165(78)90356-2.
Bacillus megaterium QM B1551 spores contained the enzymes for the Embden-Meyerhof pathway and the initial reactions in the hexose monophosphate pathway but not the Entner-Doudoroff pathway. With [U-14C]glucose no metabolism was detected until after about 30% of the spores had lost heat resistance. Mutants that lacked either phosphofructokinase or 6-phosphogluconate dehydrogenase were able to initiate germination on glucose like wild type. Purified methyl alpha-D-glucopyranoside, 6-deoxyglucose and 6-deoxy-methyl alpha-D-glucopyranoside were not substrates for spore enzymes yet these compounds still initiated germination. Therefore, metabolism of exogenously added glucose is probably not the primary stimulatory effect in germination.
巨大芽孢杆菌QM B1551孢子含有糖酵解途径的酶以及己糖磷酸途径的初始反应所需的酶，但不含有Entner-Doudoroff途径的酶。用[U-14C]葡萄糖进行实验时，直到约30%的孢子失去耐热性后才检测到代谢。缺乏磷酸果糖激酶或6-磷酸葡萄糖酸脱氢酶的突变体能够像野生型一样在葡萄糖上起始萌发。纯化的α-D-吡喃葡萄糖苷甲酯、6-脱氧葡萄糖和6-脱氧-α-D-吡喃葡萄糖苷甲酯不是孢子酶的底物，但这些化合物仍能起始萌发。因此，外源添加葡萄糖的代谢可能不是萌发过程中的主要刺激作用。
