Racine F M, Dills S S, Vary J C
J Bacteriol. 1979 May;138(2):442-5. doi: 10.1128/jb.138.2.442-445.1979.
Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germination, and then the continuous presence of glucose is not necessary. Detectable uptake of glucose began 2 to 3 min after absorbance loss started, and a non-metabolizable glucose analog, methyl-alpha-D-glucopyranoside, triggered germination in the absence of detectable uptake. Several inhibitors that reduced or eliminated glucose uptake did not block triggering of germination. Therefore, glucose uptake may be a relatively late event and not a prerequisite for triggering of germination.
研究了用 D-葡萄糖触发巨大芽孢杆菌 QM B1551 孢子萌发的情况。首先，葡萄糖与孢子相互作用不到 1 分钟，在通过稀释去除葡萄糖后，几乎 90%的孢子被触发萌发。因此，葡萄糖只需短暂时间就能触发萌发，随后葡萄糖的持续存在并非必要条件。在吸光度开始下降 2 至 3 分钟后开始检测到葡萄糖的摄取，一种不可代谢的葡萄糖类似物甲基-α-D-吡喃葡萄糖苷在未检测到摄取的情况下触发了萌发。几种降低或消除葡萄糖摄取的抑制剂并未阻止萌发的触发。因此，葡萄糖摄取可能是相对较晚发生的事件，并非触发萌发的先决条件。
