Houang E T, Greenwood D
J Clin Pathol. 1977 Aug;30(8):738-44. doi: 10.1136/jcp.30.8.738.
Ten strains each of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa were habituated to gentamicin by serial passage in antibiotic containing medium. Complete cross-resistance to streptomycin, neomycin, kanamycin, and tobramycin in a linear proportional fashion was demonstrated at all stages of habituation. Most strains of Staph. aureus and Ps. aeruginosa showed a greater increase in resistance to gentamicin than to the other three aminoglycosides. E. coli required more transfers to reach the same degree of resistance than did the other two species. Reversion to greater susceptibility to gentamicin took place after serial passage on antibiotic-free media. 'Wild' gentamicin-resistant strains showed no such proportionality of resistance to kanamycin, neomycin, or streptomycin. But many of these strains showed a proportional increase in resistance to tobramycin.
通过在含抗生素的培养基中连续传代，使10株大肠杆菌、10株金黄色葡萄球菌和10株铜绿假单胞菌适应庆大霉素。在适应的各个阶段，均显示出对链霉素、新霉素、卡那霉素和妥布霉素呈线性比例的完全交叉耐药性。大多数金黄色葡萄球菌和铜绿假单胞菌菌株对庆大霉素的耐药性增加幅度大于对其他三种氨基糖苷类抗生素的增加幅度。与其他两个菌种相比，大肠杆菌达到相同耐药程度需要更多的传代次数。在无抗生素培养基上连续传代后，对庆大霉素的敏感性恢复。“野生”庆大霉素耐药菌株对卡那霉素、新霉素或链霉素没有这种耐药比例关系。但其中许多菌株对妥布霉素的耐药性呈比例增加。
