Vince R, Weiss D, Pestka S
Antimicrob Agents Chemother. 1976 Jan;9(1):131-6. doi: 10.1128/AAC.9.1.131.
Several N-substituted erythromycylamines were evaluated for their ability to inhibit the binding of [(14)C]erythromycin to ribosomes. The association and dissociation constants for the binding of each compound to Escherichia coli ribosomes were determined. These studies have resulted in the development of three types of probes for topological studies of the erythromycin-binding site and the ribosome: the chemically reactive bromoacetamido, the photoreactive N-(2)-nitro-4-azidophenyl)glycinamido, and the fluorescent fluorescein isothiocyanate derivatives of 9(S)-erythromycylamine.
对几种N-取代的红霉素胺进行了评估，以确定它们抑制[(14)C]红霉素与核糖体结合的能力。测定了每种化合物与大肠杆菌核糖体结合的缔合常数和解离常数。这些研究促成了三种用于红霉素结合位点和核糖体拓扑学研究的探针的开发：化学反应性的溴乙酰胺基、光反应性的N-(2-硝基-4-叠氮苯基)甘氨酰胺基，以及9(S)-红霉素胺的荧光异硫氰酸荧光素衍生物。
