用于评估红霉素衍生物与核糖体结合的荧光测定法。
Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes.
作者信息
Brandt-Rauf P, Vince R, LeMahieu R, Pestka S
出版信息
Antimicrob Agents Chemother. 1978 Jul;14(1):88-94. doi: 10.1128/AAC.14.1.88.
Abstract
A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium. Results correlate well with measurements made by other techniques.
摘要
一种荧光红霉素衍生物被用于测定红霉素衍生物与核糖体的结合。该测定方法快速、灵敏且方便。由于测量是在溶液中进行的，它们代表平衡条件，这与会干扰平衡的滤膜结合测定不同。结果与通过其他技术进行的测量结果相关性良好。
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本文引用的文献
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Inhibition of [14C]chloramphenicol binding to Escherichia coli ribosomes by erythromycin derivatives.
Antimicrob Agents Chemother. 1974 Jul;6(1):39-45. doi: 10.1128/AAC.6.1.39.
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Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes.
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The distance between two functionally significant regions of the 50 S Escherichia coli ribosome: the erythromycin binding site and proteins L7/L12.
J Mol Biol. 1976 Sep 15;106(2):297-313. doi: 10.1016/0022-2836(76)90087-5.
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Binding of N-substituted erythromycyclamines to ribosomes.
Antimicrob Agents Chemother. 1976 Jan;9(1):131-6. doi: 10.1128/AAC.9.1.131.
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J Med Chem. 1977 May;20(5):732-6. doi: 10.1021/jm00215a025.
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Biochemistry. 1977 May 31;16(11):2349-56. doi: 10.1021/bi00630a007.
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