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用于评估红霉素衍生物与核糖体结合的荧光测定法。

Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes.

作者信息

Brandt-Rauf P, Vince R, LeMahieu R, Pestka S

出版信息

Antimicrob Agents Chemother. 1978 Jul;14(1):88-94. doi: 10.1128/AAC.14.1.88.

DOI:10.1128/AAC.14.1.88
PMID:356727
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC352409/
Abstract

A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium. Results correlate well with measurements made by other techniques.

摘要

一种荧光红霉素衍生物被用于测定红霉素衍生物与核糖体的结合。该测定方法快速、灵敏且方便。由于测量是在溶液中进行的，它们代表平衡条件，这与会干扰平衡的滤膜结合测定不同。结果与通过其他技术进行的测量结果相关性良好。

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1
Fluorescent assay for estimating the binding of erythromycin derivatives to ribosomes.用于评估红霉素衍生物与核糖体结合的荧光测定法。
Antimicrob Agents Chemother. 1978 Jul;14(1):88-94. doi: 10.1128/AAC.14.1.88.
2
Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes.红霉素类似物对[14C]红霉素与大肠杆菌核糖体结合的影响。
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本文引用的文献

1
Inhibition of [14C]chloramphenicol binding to Escherichia coli ribosomes by erythromycin derivatives.红霉素衍生物对[14C]氯霉素与大肠杆菌核糖体结合的抑制作用。
Antimicrob Agents Chemother. 1974 Jul;6(1):39-45. doi: 10.1128/AAC.6.1.39.
2
Effect of leucomycins and analogues on binding [14C ]erythromycin to Escherichia coli ribosomes.柱晶白霉素及其类似物对[14C]红霉素与大肠杆菌核糖体结合的影响。
Antimicrob Agents Chemother. 1974 Nov;6(5):606-12. doi: 10.1128/AAC.6.5.606.
3
Studies on the formation of transfer ribonucleic acid-ribosome complexes. 3. The formation of peptide bonds by ribosomes in the absence of supernatant enzymes.转移核糖核酸-核糖体复合物形成的研究。3. 无上清液酶时核糖体形成肽键的过程。
J Biol Chem. 1968 May 25;243(10):2810-20.
4
Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes.转移核糖核酸-核糖体复合物形成的研究。二十四。抗生素对氨酰寡核苷酸与核糖体结合的影响。
J Biol Chem. 1973 Feb 25;248(4):1168-74.
5
Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes.红霉素类似物对[14C]红霉素与大肠杆菌核糖体结合的影响。
Antimicrob Agents Chemother. 1974 Oct;6(4):479-88. doi: 10.1128/AAC.6.4.479.
6
Aromatic esters of 5-O-desosaminylerythronolide A oxime.5-O-去氧氨基红霉内酯A肟的芳香酯类
J Med Chem. 1975 Aug;18(8):849-51. doi: 10.1021/jm00242a020.
7
The distance between two functionally significant regions of the 50 S Escherichia coli ribosome: the erythromycin binding site and proteins L7/L12.
J Mol Biol. 1976 Sep 15;106(2):297-313. doi: 10.1016/0022-2836(76)90087-5.
8
Binding of N-substituted erythromycyclamines to ribosomes.N-取代红霉素胺与核糖体的结合。
Antimicrob Agents Chemother. 1976 Jan;9(1):131-6. doi: 10.1128/AAC.9.1.131.
9
Structure-activity relationships among the O-acyl derivatives of leucomycin. Correlation of minimal inhibitory concentrations with binding to Escherichia coli ribosomes.柱晶白霉素O-酰基衍生物的构效关系。最低抑菌浓度与结合大肠杆菌核糖体的相关性。
J Med Chem. 1977 May;20(5):732-6. doi: 10.1021/jm00215a025.
10
Interaction between the erythromycin and chloramphenicol binding sites on the Escherichica coli ribosome.红霉素与氯霉素在大肠杆菌核糖体上结合位点之间的相互作用。
Biochemistry. 1977 May 31;16(11):2349-56. doi: 10.1021/bi00630a007.