Hanauer L B, Christian C L
J Clin Invest. 1967 Mar;46(3):400-8. doi: 10.1172/JCI105541.
Cryoproteins observed in patients with systemic lupus erythematosus were shown to be of "mixed type," consisting largely of IgG and IgM. The IgM moiety possessed anti-IgG globulin reactivity and precipitated in the cold after mixing with a source of IgG. The IgM fraction of one cryoprotein precipitated only with the same patient's IgG. Antisera prepared against purified cryoproteins regularly recognized IgM, IgG, the 11 S component of C', and alpha(2)-macroglobulin. C'4 (beta(1E)) and C'3 (beta(1C)) were recognized by four and two antisera, respectively. Antisera prepared against two cryoproteins reacted (after absorption) only with the sera of origin. These "unique" antigens were associated with IgM, were destroyed by mercaptoethanol treatment, and, in one patient, were shown to disappear subsequent to clinical improvement.
在系统性红斑狼疮患者中观察到的冷球蛋白显示为“混合型”，主要由IgG和IgM组成。IgM部分具有抗IgG球蛋白反应性，并在与IgG来源混合后在低温下沉淀。一种冷球蛋白的IgM部分仅与同一患者的IgG沉淀。针对纯化冷球蛋白制备的抗血清通常能识别IgM、IgG、补体C'的11S成分和α2-巨球蛋白。C'4（β1E）和C'3（β1C）分别被四种和两种抗血清识别。针对两种冷球蛋白制备的抗血清（吸收后）仅与原始血清反应。这些“独特”抗原与IgM相关，经巯基乙醇处理后被破坏，并且在一名患者中，显示在临床改善后消失。
