病理免疫球蛋白G重链的溴化氰裂解及部分序列分析
Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G.
作者信息
Piggot P J, Press E M
出版信息
Biochem J. 1967 Aug;104(2):616-26. doi: 10.1042/bj1040616.
Abstract
The heavy chain of a pathological immunoglobulin G (Daw) of type L, subclass gamma(2b) (We) and Gm(a+)(f-), has been cleaved with cyanogen bromide. The five fragments resulting from the cleavage have been isolated and analysed. The papain-digest fragments, Fab and Fc, have also been cleaved with cyanogen bromide and the products analysed and compared with those from the heavy chain. The order of the five fragments in the heavy chain has been established and the location of some of the inter-chain and inter-fragment disulphide bonds has been determined. The sequence of the N-terminal fragment consisting of 34 residues is reported.
摘要
L型、γ(2b)亚类（We）且Gm(a+)(f-)的病理性免疫球蛋白G（Daw）的重链已用溴化氰裂解。已分离并分析了裂解产生的五个片段。木瓜蛋白酶消化片段Fab和Fc也用溴化氰裂解，并对产物进行分析，与重链裂解产物进行比较。已确定重链中五个片段的顺序，并确定了一些链间和片段间二硫键的位置。报道了由34个残基组成的N端片段的序列。
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