类风湿性滑膜细胞在组织培养中导致软骨基质消耗。
Cartilage matrix depletion by rheumatoid synovial cells in tissue culture.
作者信息
Hamerman D, Janis R, Smith C
出版信息
J Exp Med. 1967 Dec 1;126(6):1005-12. doi: 10.1084/jem.126.6.1005.
Abstract
Articular cartilage fragments were added to monolayer cultures of synovial membrane cells. After 3 wk of incubation, the cartilage fragments were examined histologically for metachromasia and basophilia, and for fluorescent staining using a rabbit antiserum to cartilage protein-polysaccharide. Cartilage incubated with cells derived from rheumatoid synovial membranes showed striking loss of metachromasia and basophilia as well as diminished to absent fluorescent staining. Cartilage fragments incubated with cells from normal synovia, or with cells from the synovial membrane of a patient with Reiter's syndrome, did not show these changes and resembled control cartilage incubated in tissue culture medium alone. It appears, therefore, that rheumatoid synovial cells in tissue culture are able to deplete the matrix of articular cartilage.
摘要
将关节软骨碎片添加到滑膜细胞的单层培养物中。培养3周后，对软骨碎片进行组织学检查，观察异染性和嗜碱性，并使用兔抗软骨蛋白多糖抗血清进行荧光染色。与类风湿性滑膜炎来源的细胞一起培养的软骨显示出明显的异染性和嗜碱性丧失，以及荧光染色减弱或消失。与正常滑膜细胞或赖特综合征患者滑膜细胞一起培养的软骨碎片未出现这些变化，且与仅在组织培养基中培养的对照软骨相似。因此，似乎组织培养中的类风湿性滑膜细胞能够消耗关节软骨的基质。
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