Asherson G L, Ptak W
Immunology. 1968 Sep;15(3):405-16.
Ear swelling in mice was measured with a micrometer and used to quantify 24-hour skin reactions. Specific contact sensitivity occurred in mice immunized with picryl chloride, 2-phenyl-4-ethoxymethylene oxazolone, dinitrofluorobenzene and tetramethyl--phenylenediamine as shown by ear swelling 24 hours after challenge. In some mice sensitized with oxazolone significant swelling occurred 4 hours after challenge. It was possible to induce tolerance in adult mice and contact sensitivity to dinitrochlorobenzene was diminished by prior treatment with dinitrobenzenesulphonic acid. There was some evidence of delayed hypersensitivity to protein antigens. Twenty-four-hour skin reactions to PPD (purified protein derivative of tuberculin) occurred in mice immunized with live BCG or dead tubercle bacilli in Freund's adjuvant. In most experiments the swelling at 24 hours was greater than at 4 hours. Similar reactions to egg albumin and bovine γ-globulin were seen in mice immunized with these antigens in Freund's complete adjuvant and one group of mice showed greater swelling at 24 hours after challenge than at 4 hours. Contact sensitivity was readily transferred in inbred mice by peritoneal exudate cells when the challenge was undertaken immediately after transfer. Variable and usually inferior passive transfer was obtained with lymph node cells. Attempts to transfer contact sensitivity in outbred mice were unsuccessful.
用测微计测量小鼠耳部肿胀，并用于量化24小时的皮肤反应。在用苦味酰氯、2-苯基-4-乙氧基亚甲基恶唑酮、二硝基氟苯和四甲基对苯二胺免疫的小鼠中出现了特异性接触敏感性，如在激发后24小时耳部肿胀所示。在用恶唑酮致敏的一些小鼠中，激发后4小时出现明显肿胀。在成年小鼠中诱导耐受性是可能的，并且用二硝基苯磺酸预先处理可降低对二硝基氯苯的接触敏感性。有一些证据表明对蛋白质抗原有迟发型超敏反应。在用活卡介苗或弗氏佐剂中的死结核杆菌免疫的小鼠中，出现了对PPD（结核菌素纯蛋白衍生物）的24小时皮肤反应。在大多数实验中，24小时时的肿胀大于4小时时的肿胀。在用弗氏完全佐剂中的这些抗原免疫的小鼠中，观察到对卵白蛋白和牛γ球蛋白的类似反应，并且一组小鼠在激发后24小时时的肿胀大于4小时时的肿胀。当在转移后立即进行激发时，接触敏感性很容易通过腹膜渗出细胞在近交系小鼠中传递。用淋巴结细胞进行被动转移时，结果不一，通常效果较差。在远交系小鼠中转移接触敏感性的尝试未成功。
