Richardson U I, Tashjian A H, Levine L
J Cell Biol. 1969 Jan;40(1):236-47. doi: 10.1083/jcb.40.1.236.
A clonal strain of epithelial cells (designated MH(1)C(1)) has been established from the transplantable Morris hepatoma No. 7795. The cells have maintained distinctive morphology throughout more than 20 subcultures (split 1:5) at 2- to 4-week intervals in supplemented Ham's F 10 medium. They contain many highly refractile, round, cytoplasmic bodies which stain bright red with Oil Red O. The population doubling time was 2 wk when the clonal strain was first established. It has gradually decreased to 1 wk. The cells synthesize rat serum albumin and secrete it into the culture medium as determined immunologically by microcomplement fixation and double diffusion. Albumin secretion (3-6 microg albumin/mg cell nitrogen/24 hr) occurs throughout the logarithmic phase of cell proliferation and has not diminished during serial propagation since the strain was initiated 15 months ago.
已从可移植的莫里斯7795号肝癌中建立了一株上皮细胞克隆株(命名为MH(1)C(1))。在添加了Ham's F 10培养基中,细胞每隔2至4周以1:5的比例传代培养20多次后,仍保持独特的形态。它们含有许多高度折光的圆形细胞质小体,用苏丹红O染色呈鲜红色。克隆株刚建立时群体倍增时间为2周,现已逐渐降至1周。通过微量补体结合和双向扩散免疫测定法确定,这些细胞能合成大鼠血清白蛋白并将其分泌到培养基中。白蛋白分泌(3 - 6微克白蛋白/毫克细胞氮/24小时)在细胞增殖的对数期全程发生,自该株系15个月前建立以来,在连续传代过程中并未减少。