Blanchard G C, Gardner R
Clin Chem. 1978 May;24(5):808-14.
We found high correlations when the values for immunoglobulins G, A, and M, obtained from the analysis of a group of 50 or more human sera by a measured-time radial immunodiffusion method, were compared to values found by two new commercial immunofluorescent methods. The Fiax and the Immuno-Fluor systems make use of fluorescently labeled antibodies and solid-phase immunoadsorbents and offer simple, precise, and objective methods for immunoglobulin quantitation. The Fiax procedure is less time consuming than is the Immuno-Fluor and gives IgG and IgA concentrations that are in close agreement with those found by radial immunodiffusion and IgM values that are significantly lower than the radial immunodiffusion values. The IgG results determined by the Immuno-Fluor method compare well with the radial immunodiffusion results, uhile the IgA values are significantly higher and IgM values significantly lower than those obtained by radial immunodiffusion.
当通过定时放射免疫扩散法对一组50份或更多份人血清进行分析得到的免疫球蛋白G、A和M的值，与两种新的商业免疫荧光法得到的值进行比较时，我们发现了高度相关性。Fiax和免疫荧光系统利用荧光标记抗体和固相免疫吸附剂，为免疫球蛋白定量提供了简单、精确且客观的方法。Fiax方法比免疫荧光方法耗时少，其给出的IgG和IgA浓度与放射免疫扩散法得到的浓度非常一致，而IgM值则明显低于放射免疫扩散法得到的值。免疫荧光法测定的IgG结果与放射免疫扩散结果相当，而IgA值明显更高，IgM值明显低于放射免疫扩散法得到的值。
