一种佐剂活性测定法。
An assay for adjuvanticity.
作者信息
Dresser D W
出版信息
Clin Exp Immunol. 1968 Nov;3(9):877-88.
Adult mice injected with an adequate amount of a non-immunogenic antigen progress to a specific state of immunological paralysis, unless a substance with `extrinsic' adjuvanticity is injected before the induction of paralysis is completed. Consequently incipiently paralysed mice can be used to assay substances for adjuvanticity. Conventional adjuvants such as Freund's adjuvant and pertussis possess adjuvanticity; other substances with varying degrees of adjuvanticity are listed in the tables. It has been shown that the adjuvanticity effect of an injection of pertussis lasts for only a few days, although the effect of such an injection of pertussis on phagocytosis of carbon particles does not reach a maximum until 2 weeks after the injection. The dose-effectiveness of alum precipitated (highly phagocytosable) bovine γ-globulin was greatly increased by the intraperitoneal injection of pertussis. The evidence is considered to be incompatible with increased phagocytosis being either an essential factor in the role of pertussis as a conventional adjuvant, or in the adjuvanticity effect of pertussis.
给成年小鼠注射适量的非免疫原性抗原，小鼠会发展到一种特定的免疫麻痹状态，除非在麻痹诱导完成前注射具有“外源性”佐剂活性的物质。因此，处于初期麻痹状态的小鼠可用于检测物质的佐剂活性。传统佐剂如弗氏佐剂和百日咳菌苗具有佐剂活性；表中列出了其他具有不同程度佐剂活性的物质。已经表明，注射百日咳菌苗的佐剂活性作用仅持续几天，尽管这种注射百日咳菌苗对碳颗粒吞噬作用的影响直到注射后2周才达到最大值。通过腹腔注射百日咳菌苗，明矾沉淀（高度可吞噬）的牛γ球蛋白的剂量效应大大增加。有证据表明，吞噬作用增强既不是百日咳菌苗作为传统佐剂发挥作用的关键因素，也不是百日咳菌苗佐剂活性作用的关键因素。
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