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小鼠浆细胞瘤游离核糖体对免疫球蛋白重链和轻链的合成

Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.

作者信息

Lisowska-Bernstein B, Lamm M E, Vassalli P

出版信息

Proc Natl Acad Sci U S A. 1970 Jun;66(2):425-32. doi: 10.1073/pnas.66.2.425.

DOI:10.1073/pnas.66.2.425
PMID:4194867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283062/
Abstract

Free and membrane-bound ribosomes have been isolated from a mouse plasma cell tumor secreting immunoglobulins, and allowed to synthesize proteins under cell-free conditions. Analysis of the products of synthesis by autoradiography of immunoelectrophoresis and peptide maps indicates that both types of ribosomes synthesize heavy and light immunoglobulin chains.

摘要

已从分泌免疫球蛋白的小鼠浆细胞瘤中分离出游离核糖体和膜结合核糖体，并使其在无细胞条件下合成蛋白质。通过免疫电泳放射自显影和肽图对合成产物进行分析表明，这两种类型的核糖体均能合成重链和轻链免疫球蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/715d5b5b652f/pnas00097-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/333b9d03ac63/pnas00097-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/218ea6a83e91/pnas00097-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/715d5b5b652f/pnas00097-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/333b9d03ac63/pnas00097-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/218ea6a83e91/pnas00097-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7b8/283062/715d5b5b652f/pnas00097-0184-a.jpg

相似文献

1
Synthesis of immunoglobulin heavy and light chains by the free ribosomes of a mouse plasma cell tumor.小鼠浆细胞瘤游离核糖体对免疫球蛋白重链和轻链的合成
Proc Natl Acad Sci U S A. 1970 Jun;66(2):425-32. doi: 10.1073/pnas.66.2.425.
2
Cell-free synthesis of rat immunoglobulin. 3. Analysis of the cell-free made chains and of their mode of assembly.大鼠免疫球蛋白的无细胞合成。3. 对无细胞合成的链及其组装方式的分析。
J Mol Biol. 1971 Feb 28;56(1):1-19. doi: 10.1016/0022-2836(71)90080-5.
3
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Arch Biochem Biophys. 1968 May;125(2):401-9. doi: 10.1016/0003-9861(68)90596-1.
4
Electron microscopy of polyribosomes synthesizing immunoglobulin chains. Comparison with sizes determined by density-gradient-sedimentation data.
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5
Chemical identification of specific immunoglobulins as the product of a cell-free system from plasmocytoma tumors.将特定免疫球蛋白化学鉴定为来自浆细胞瘤肿瘤的无细胞系统的产物。
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A protein related to immunoglobulin light chain detected in mouse myeloma cells.在小鼠骨髓瘤细胞中检测到的一种与免疫球蛋白轻链相关的蛋白质。
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引用本文的文献

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Protein Synthesis and Translational Control: A Historical Perspective.蛋白质合成与翻译调控：历史透视。
Cold Spring Harb Perspect Biol. 2019 Sep 3;11(9):a035584. doi: 10.1101/cshperspect.a035584.
2
Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomes.骨髓瘤细胞的膜结合核糖体。IV. 游离及膜结合多核糖体的mRNA复杂性
J Cell Biol. 1981 Jan;88(1):29-36. doi: 10.1083/jcb.88.1.29.
3
Immunoglobulin synthesis and secretion. VI. Synthesis and intracellular transport of immunoglobulin in nonsecretory lymphoma cells.

本文引用的文献

1
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.豚鼠胰腺的细胞化学研究。5. 亮氨酸-1-C14在体内掺入各种细胞组分的糜蛋白酶原中的情况。
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HERITABLE VARIATION IN BENCE JONES PROTEIN STRUCTURE IN AN INBRED STRAIN OF MICE.近交系小鼠中本-周蛋白结构的可遗传变异
J Mol Biol. 1964 Jun;8:814-22. doi: 10.1016/s0022-2836(64)80162-5.
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Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.
免疫球蛋白的合成与分泌。VI. 非分泌性淋巴瘤细胞中免疫球蛋白的合成及细胞内转运
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Immunoglobulin synthesis and secretion. V. Incorporation of leucine and glucosamine into immunoglobulin on free and bound polyribosomes.免疫球蛋白的合成与分泌。V. 亮氨酸和氨基葡萄糖掺入游离及结合多核糖体上的免疫球蛋白的情况。
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1183-9. doi: 10.1073/pnas.66.4.1183.
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Insulin biosynthesis: studies of Islet polyribosomes (nascent peptides-sucrose gradient analysis-gel filtration).胰岛素生物合成：胰岛多核糖体的研究（新生肽-蔗糖梯度分析-凝胶过滤）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):505-9. doi: 10.1073/pnas.69.2.505.
6
Biologically and chemically pure mRNA coding for a mouse immunoglobulin L-chain prepared with the aid of antibodies and immobilized oligothymidine.借助抗体和固定化寡聚胸腺嘧啶制备的编码小鼠免疫球蛋白轻链的生物和化学纯mRNA。
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2256-60. doi: 10.1073/pnas.70.8.2256.
7
Purification of 14S messenger RNA of immunoglobulin light chain that codes for a possible light-chain precursor.编码一种可能的轻链前体的免疫球蛋白轻链14S信使核糖核酸的纯化。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):451-5. doi: 10.1073/pnas.70.2.451.
8
Purification and properties of biologically active messenger RNA for a myeloma light chain.骨髓瘤轻链生物活性信使核糖核酸的纯化及特性
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1967-71. doi: 10.1073/pnas.69.7.1967.
9
Role of membrane-bound and free polyribosomes in the synthesis of cytochrome c in rat liver.膜结合多核糖体和游离多核糖体在大鼠肝脏细胞色素c合成中的作用
Biochem J. 1974 May;140(2):157-67. doi: 10.1042/bj1400157.
10
Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses.在复制小鼠肉瘤-白血病病毒的细胞的多核糖体中的病毒特异性信使核糖核酸和新生多肽。
Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064-8. doi: 10.1073/pnas.70.7.2064.
大鼠肝脏中的核糖体：体内游离核糖体和膜结合核糖体与信使核糖核酸相互作用的百分比估算
J Mol Biol. 1967 Sep 28;28(3):539-42. doi: 10.1016/s0022-2836(67)80103-7.
4
Isolation and properties of polyribosomes and fragments of the endoplasmic reticulum from rat liver.大鼠肝脏多核糖体和内质网片段的分离及特性
Biochem J. 1967 Apr;103(1):177-82. doi: 10.1042/bj1030177.
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Free and membrane-bound ribosomes in rat liver.大鼠肝脏中的游离核糖体和附着核糖体。
J Biol Chem. 1967 May 10;242(9):2069-74.
6
Biosynthesis of serum proteins and ferritin by free and attached ribosomes of rat liver.大鼠肝脏游离核糖体和附着核糖体对血清蛋白及铁蛋白的生物合成
J Biol Chem. 1969 Aug 25;244(16):4308-15.
7
Preferential synthesis of ferritin and albumin by different populations of liver polysomes.肝脏多核糖体不同群体对铁蛋白和白蛋白的优先合成。
Science. 1969 May 2;164(3879):584-5. doi: 10.1126/science.164.3879.584.
8
Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes.大鼠肝细胞组分中新生多肽的可控蛋白水解作用。II. 多肽在糙面微粒体中的定位
J Cell Biol. 1970 Apr;45(1):146-57. doi: 10.1083/jcb.45.1.146.
9
Vectorial discharge of peptides released by puromycin from attached ribosomes.嘌呤霉素从附着核糖体释放的肽的向量性排出。
Proc Natl Acad Sci U S A. 1966 Aug;56(2):608-15. doi: 10.1073/pnas.56.2.608.
10
Studies on cell-free synthesis of rat immunoglobulins, II. Synthesis of immunoglobulin and of antibody to the dinitrophenyl hapten.大鼠免疫球蛋白的无细胞合成研究，II。免疫球蛋白及二硝基苯基半抗原抗体的合成。
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2422-9. doi: 10.1073/pnas.58.6.2422.