Le Goffic F, Andrillon-Spiegel J, Letarte R
Antimicrob Agents Chemother. 1974 Dec;6(6):676-9. doi: 10.1128/AAC.6.6.676.
A beta-lactamase was extracted from an Escherichia coli K-12 strain carrying the R-TEM plasmid and has been purified by affinity chromatography. Antisera to this enzyme were prepared in the rabbit, and the enzyme-antibody neutralization reaction has been evaluated with acidimetric methods (pH stat or pH meter). Under defined experimental conditions, it is now possible to clearly illustrate the enzyme-antibody reaction by means of accurate, rapid, and simple-to-perform methods. These methods are in accord with the specificity of the two reactive partners and allow the detection of the enzyme in a crude bacterial extract which would eventually contain more than one beta-lactamase.
从携带R-TEM质粒的大肠杆菌K-12菌株中提取了一种β-内酰胺酶，并通过亲和色谱法进行了纯化。用兔制备了针对该酶的抗血清，并用酸度测定法（pH计或pH-stat）评估了酶-抗体中和反应。在确定的实验条件下，现在可以通过准确、快速且易于操作的方法清楚地阐明酶-抗体反应。这些方法符合两种反应伙伴的特异性，并允许在最终可能含有不止一种β-内酰胺酶的粗细菌提取物中检测该酶。
