McIntosh K, McQuillin J, Gardner P S
Infect Immun. 1979 Feb;23(2):276-81. doi: 10.1128/iai.23.2.276-281.1979.
Twenty-two infants under 9 months of age hospitalized with bronchiolitis or pneumonia due to respiratory syncytial virus (RSV) were serially sampled to determine the pattern of secretory antibody response. Using double labeling techniques, we found several types of immunoglobulin in secretions: cell-free antibody to RSV of the immunoglobulin A (IgA), immunoglobulin G (IgG), and immunoglobulin M (IgM) classes; and immunoglobulins of all three classes bound to RSV-infected cells shed from the nasal epithelium (presumably cell-bound antibody to RSV). IgA attached to RSV-infected epithelial cells was almost always detected in the first available nasal sample (day 1 or 2 of hospitalization). In contrast, cell-free anti-RSV IgA first appeared an average of 3.5 days later at a time when virus antigen was disappearing from the secretion. IgG and IgM attached to RSV-infected cells appeared more irregularly. The titer of cell-free anti-RSV IgM was often higher than that of IgA early in the illness and declined as the infection resolved. Cell-free anti-RSV IgG was usually present earlier than IgA and rose during convalescence.
对22名因呼吸道合胞病毒（RSV）导致细支气管炎或肺炎而住院的9个月龄以下婴儿进行了系列采样，以确定分泌性抗体反应模式。使用双重标记技术，我们在分泌物中发现了几种免疫球蛋白：免疫球蛋白A（IgA）、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）类别的游离RSV抗体；以及与从鼻上皮脱落的RSV感染细胞结合的所有三类免疫球蛋白（推测为与RSV结合的细胞抗体）。附着在RSV感染上皮细胞上的IgA几乎总是在首次获得的鼻样本（住院第1天或第2天）中检测到。相比之下，游离抗RSV IgA平均在3.5天后首次出现，此时病毒抗原正从分泌物中消失。附着在RSV感染细胞上的IgG和IgM出现得更不规律。在疾病早期，游离抗RSV IgM的滴度通常高于IgA，并且随着感染的消退而下降。游离抗RSV IgG通常比IgA更早出现，并在恢复期上升。
