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通过膜荧光检测呼吸道合胞病毒抗体

Detection of antibody to respiratory syncytial virus by membrane fluorescence.

作者信息

Scott R, De Landazuri M O, Gardner P S, Owen J J

出版信息

Clin Exp Immunol. 1976 Oct;26(1):78-85.

PMID:793751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1540801/
Abstract

An indirect membrane fluorescent antibody technique was established to study HEp 2 cells infected with respiratory syncytial (RS) virus. It was possible to detect IgG and IgM antibody to RS virus in the sera of patients with respiratory infections using this technique. The technique was further applied to the detection of IgA antibody to the same virus in colostrum.

摘要

建立了一种间接膜荧光抗体技术来研究感染呼吸道合胞（RS）病毒的人喉表皮癌细胞（HEp 2细胞）。使用该技术能够检测呼吸道感染患者血清中针对RS病毒的IgG和IgM抗体。该技术进一步应用于检测初乳中针对同一病毒的IgA抗体。

相似文献

1
Detection of antibody to respiratory syncytial virus by membrane fluorescence.通过膜荧光检测呼吸道合胞病毒抗体
Clin Exp Immunol. 1976 Oct;26(1):78-85.
2
An attempt to determine the relation between levels of IgG, IgA and IgM immunoglobulins and titers of antibodies against RS virus.一项旨在确定IgG、IgA和IgM免疫球蛋白水平与抗呼吸道合胞病毒抗体滴度之间关系的研究。
Arch Immunol Ther Exp (Warsz). 1977;25(4):477-82.
3
Human antibody-dependent cell-mediated cytotoxicity against target cells infected with respiratory syncytial virus.人类针对感染呼吸道合胞病毒的靶细胞的抗体依赖性细胞介导的细胞毒性作用。
Clin Exp Immunol. 1977 Apr;28(1):19-26.
4
An enzyme-linked immunosorbent assay (ELISA) for IgG and IgA antibodies to respiratory syncytial virus in low dilutions of secretions of human serum and secretions.一种用于检测人血清和分泌物低稀释度中呼吸道合胞病毒IgG和IgA抗体的酶联免疫吸附测定（ELISA）。
J Med Virol. 1984;14(3):285-94. doi: 10.1002/jmv.1890140313.
5
In vitro cell-dependent lysis of respiratory syncytial virus-infected cells mediated by antibody from local respiratory secretions.由局部呼吸道分泌物中的抗体介导的呼吸道合胞病毒感染细胞的体外细胞依赖性裂解。
Clin Exp Immunol. 1981 Jan;43(1):28-35.
6
Detection of respiratory syncytial virus serum antibodies by an ELISA system.用酶联免疫吸附测定系统检测呼吸道合胞病毒血清抗体。
Acta Virol. 1981 Jan;25(1):41-8.
7
Immunity to infections of the respiratory system in man and animals.人和动物对呼吸系统感染的免疫力。
Dev Biol Stand. 1975;28:609-16.
8
The coating of respiratory syncytial (RS) virus-infected cells in the respiratory tract by immunoglobulins.呼吸道中免疫球蛋白对呼吸道合胞（RS）病毒感染细胞的包被。
J Med Virol. 1978;2(2):165-73. doi: 10.1002/jmv.1890020211.
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[Evaluation of serological examination in the diagnosis of RS virus infection in infants].[血清学检查在婴幼儿呼吸道合胞病毒感染诊断中的评估]
Med Dosw Mikrobiol. 1988;40(3):167-72.
10
[Early diagnosis of respiratory syncytial virus infection in infants and young children. Detection of specific IgM by indirect immunofluorescence technic].[婴幼儿呼吸道合胞病毒感染的早期诊断。用间接免疫荧光技术检测特异性IgM]
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1982 Dec;4(6):388-91.

引用本文的文献

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Cryopreservation of virus-infected cells for use in the fluorescent antibody to membrane antigen test.用于膜抗原荧光抗体检测的病毒感染细胞的冷冻保存。
J Clin Microbiol. 1980 Jun;11(6):687-90. doi: 10.1128/jcm.11.6.687-690.1980.
2
Detection, pathogenesis, and therapy of respiratory syncytial virus infections.呼吸道合胞病毒感染的检测、发病机制及治疗
Clin Microbiol Rev. 1988 Jan;1(1):27-39. doi: 10.1128/CMR.1.1.27.
3
Immunity to human and bovine respiratory syncytial virus.对人及牛呼吸道合胞病毒的免疫力。
Arch Virol. 1990;112(1-2):1-25. doi: 10.1007/BF01348982.
4
Human antibody-dependent cell-mediated cytotoxicity against target cells infected with respiratory syncytial virus.人类针对感染呼吸道合胞病毒的靶细胞的抗体依赖性细胞介导的细胞毒性作用。
Clin Exp Immunol. 1977 Apr;28(1):19-26.
5
Cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial virus infection.呼吸道合胞病毒感染婴儿鼻分泌物中的游离抗体和结合细胞的抗体。
Infect Immun. 1979 Feb;23(2):276-81. doi: 10.1128/iai.23.2.276-281.1979.
6
Rapid subtyping of influenza A virus isolates by membrane fluorescence.通过膜荧光对甲型流感病毒分离株进行快速亚型分型。
J Clin Microbiol. 1979 Feb;9(2):269-73. doi: 10.1128/jcm.9.2.269-273.1979.

本文引用的文献

1
Technique of complementfixation test applicable to the diagnosis of virus diseases.适用于病毒疾病诊断的补体结合试验技术。
Mon Bull Minist Health Public Health Lab Serv. 1962 May;21:96-104.
2
Immunoglobulin spots on the surface of rabbit lymphocytes.兔淋巴细胞表面的免疫球蛋白斑点。
J Exp Med. 1970 Nov;132(5):1001-18. doi: 10.1084/jem.132.5.1001.
3
Relation between Epstein-Barr viral and cell membrane immunofluorescence of Burkitt tumor cells. I. Dependence of cell membrane immunofluorescence on presence of EB virus.伯基特肿瘤细胞的爱泼斯坦-巴尔病毒与细胞膜免疫荧光之间的关系。I. 细胞膜免疫荧光对EB病毒存在的依赖性。
J Exp Med. 1968 Nov 1;128(5):1011-20. doi: 10.1084/jem.128.5.1011.
4
Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques.通过免疫荧光抗体技术对呼吸道合胞病毒感染进行快速诊断。
Br Med J. 1968 Mar 9;1(5592):602-5. doi: 10.1136/bmj.1.5592.602.
5
IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique.用免疫荧光技术测定急性风疹中的IgG、IgA和IgM反应。
J Hyg (Lond). 1972 Sep;70(3):473-85. doi: 10.1017/s0022172400063063.
6
Antibody-induced redistribution of measles virus antigens on the cell surface.抗体诱导麻疹病毒抗原在细胞表面的重新分布。
J Immunol. 1974 Oct;113(4):1205-9.
7
Incidence and persistence of mumps-specific IgM and IgA in the sera of mumps patients.腮腺炎患者血清中腮腺炎特异性IgM和IgA的发生率及持续存在情况。
Scand J Infect Dis. 1974;6(2):125-9. doi: 10.3109/inf.1974.6.issue-2.05.
8
Virus-specific immunoglobulins in multiple sclerosis.多发性硬化症中的病毒特异性免疫球蛋白
Clin Exp Immunol. 1973 Jul;14(3):409-16.
9
IgM antibodies to Epstein-Barr virus in infectious mononucleosis.传染性单核细胞增多症中针对EB病毒的IgM抗体。
Arch Gesamte Virusforsch. 1972;37(4):332-9. doi: 10.1007/BF01241456.
10
Immunofluorescent studies of mouse and hamster cell surface antigens induced by polyoma virus.多瘤病毒诱导的小鼠和仓鼠细胞表面抗原的免疫荧光研究。
J Natl Cancer Inst. 1968 Feb;40(2):263-8.