Wilkinson B J, Peterson P K, Quie P G
Infect Immun. 1979 Feb;23(2):502-8. doi: 10.1128/iai.23.2.502-508.1979.
The antiphagocytic effect of the Staphylococcus aureus capsule is known to be related to its ability to interfere with opsonization by normal human serum. In this study, evidence is presented with isolated cell surface components which indicates that the capsule hinders opsonization by masking cell wall peptidoglycan. In contrast to intact, encapsulated S. aureus M cells, peptidoglycan particles isolated from the organism were efficiently opsonized by normal human serum and phagocytized by human polymorphonuclear leukocytes. Cell wall particles retaining capsular material were opsonized less efficiently than peptidoglycan. Studies comparing the opsonic capacities of normal, C2-deficient, and heat-inactivated sera led to the conclusion that both the classical and the alternative complement pathways contribute to the opsonization of peptidoglycan in normal human serum. It appears that the capsule interferes with opsonization via both of these complement pathways. Serum from rabbits immunized with S. aureus M had significant heat-stable opsonic activity for the intact organism and cell walls retaining capsular material, but not for peptidoglycan. A general model is proposed to explain how antiphagocytic cell surface polymers may inhibit bacterial opsonization and thereby impede natural immunity.
已知金黄色葡萄球菌荚膜的抗吞噬作用与其干扰正常人血清调理作用的能力有关。在本研究中，通过分离的细胞表面成分提供的证据表明，荚膜通过掩盖细胞壁肽聚糖来阻碍调理作用。与完整的、有荚膜的金黄色葡萄球菌M细胞不同，从该生物体分离的肽聚糖颗粒能被正常人血清有效调理并被人多形核白细胞吞噬。保留荚膜物质的细胞壁颗粒的调理效率低于肽聚糖。比较正常血清、C2缺陷血清和热灭活血清调理能力的研究得出结论，经典补体途径和替代补体途径都有助于正常人血清中肽聚糖的调理作用。看来荚膜通过这两种补体途径干扰调理作用。用金黄色葡萄球菌M免疫的兔血清对完整生物体和保留荚膜物质的细胞壁具有显著的热稳定调理活性，但对肽聚糖则没有。提出了一个通用模型来解释抗吞噬细胞表面聚合物如何抑制细菌调理作用，从而阻碍天然免疫。
