Peterson P K, Wilkinson B J, Kim Y, Schmeling D, Quie P G
Infect Immun. 1978 Mar;19(3):943-9. doi: 10.1128/iai.19.3.943-949.1978.
In previous studies, encapsulated Staphylococcus aureus strains have been shown to resist phagocytosis. In this investigation, the nature of the interference with phagocytosis by human polymorphonuclear leukocytes was examined by studying the opsonization of two pairs of unencapsulated (Smith compact and M variant) and encapsulated (Smith diffuse and M) S. aureus strains. The uptake of [3H]glycine-labeled bacteria by normal leukocytes was quantitatively measured after incubation of bacteria in pooled serum, C2-deficient serum, immunoglobulin-deficient serum, and serum from a rabbit immunized with S. aureus M. The presence of a capsule was found to interfere with opsonization by both the classical and alternative pathways of complement as well as by heat-stable opsonic factors in nonimmune human serum. This interference was significantly greater in the case of the S. aureus M strain than in the case of the Smith diffuse strain. The only effective opsonic source for S. aureus M was immune rabbit serum. It is proposed that encapsulation of S. aureus strains interferes with phagocytosis by preventing effective bacterial opsonization.
在先前的研究中，已证明被包裹的金黄色葡萄球菌菌株能够抵抗吞噬作用。在本研究中，通过研究两对未被包裹的（史密斯致密型和M变异型）及被包裹的（史密斯弥散型和M型）金黄色葡萄球菌菌株的调理作用，对人类多形核白细胞吞噬作用的干扰性质进行了研究。在将细菌分别与混合血清、C2缺陷血清、免疫球蛋白缺陷血清以及用金黄色葡萄球菌M免疫的兔子血清孵育后，定量测定正常白细胞对[3H]甘氨酸标记细菌的摄取。结果发现，荚膜的存在会干扰补体的经典途径和替代途径以及非免疫人血清中的热稳定调理因子的调理作用。这种干扰在金黄色葡萄球菌M菌株的情况下比在史密斯弥散型菌株的情况下要大得多。金黄色葡萄球菌M唯一有效的调理来源是免疫兔血清。有人提出，金黄色葡萄球菌菌株的包裹通过阻止有效的细菌调理作用来干扰吞噬作用。
