Moss B, Salzman N P
J Virol. 1968 Oct;2(10):1016-27. doi: 10.1128/JVI.2.10.1016-1027.1968.
Inhibition of HeLa cell protein synthesis and the sequential synthesis of viral proteins were followed by pulse-labeling infected cells with (14)C-phenylalanine. Proteins were resolved by polyacrylamide gel electrophoresis. The viral origin of native proteins was confirmed by immunodiffusion. The inhibition of host protein synthesis and the synthesis of early viral proteins occur 1 to 3 hr after infection. This early sequence of events also occurs in the presence of 5-fluorodeoxyuridine, an inhibitor of deoxyribonucleic acid synthesis. Other viral proteins are synthesized at a later time. Those proteins which are not made in the absence of viral deoxyribonucleic acid synthesis can be further subdivided into intermediate and late classes. The intermediate protein is synthesized before the late proteins but does not appear to be a precursor of them. Many more viral polypeptides were resolved by polyacrylamide gel electrophoresis after solubilization of the entire cytoplasmic fraction with sodium dodecyl sulfate. Virion and nonvirion proteins were identified. Kinetic experiments suggested that certain structural proteins as well as certain nonstructural proteins are made early, whereas others of both classes are made primarily at later times.
通过用（14）C-苯丙氨酸脉冲标记受感染细胞，跟踪HeLa细胞蛋白质合成的抑制以及病毒蛋白的顺序合成。蛋白质通过聚丙烯酰胺凝胶电泳进行分离。通过免疫扩散确认天然蛋白质的病毒来源。宿主蛋白质合成的抑制和早期病毒蛋白的合成在感染后1至3小时发生。这一早期事件序列在脱氧核糖核酸合成抑制剂5-氟脱氧尿苷存在的情况下也会发生。其他病毒蛋白在较晚时间合成。那些在没有病毒脱氧核糖核酸合成时不产生的蛋白质可进一步细分为中期和晚期类别。中期蛋白在晚期蛋白之前合成，但似乎不是晚期蛋白的前体。在用十二烷基硫酸钠溶解整个细胞质部分后，通过聚丙烯酰胺凝胶电泳解析出了更多的病毒多肽。鉴定出了病毒粒子和非病毒粒子蛋白。动力学实验表明，某些结构蛋白以及某些非结构蛋白在早期产生，而这两类中的其他蛋白主要在较晚时间产生。
