从人软骨制备不溶性胶原蛋白。
Preparation of insoluble collagen from human cartilage.
作者信息
Steven F S, Thomas H
出版信息
Biochem J. 1973 Sep;135(1):245-7. doi: 10.1042/bj1350245.
Abstract
A simple technique is described for the removal of proteoglycans from human cartilage by sequential treatments with H(2)O(2) followed by trypsin digestion. The resultant fibres contain no uronic acids and have the amino acid composition of highly purified collagen.
摘要
本文描述了一种简单的技术，通过先用H₂O₂处理，然后进行胰蛋白酶消化，从人软骨中去除蛋白聚糖。所得纤维不含糖醛酸，且具有高度纯化胶原蛋白的氨基酸组成。
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