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Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased.

作者信息

Bell L, Williams L

出版信息

Histochemistry. 1979 Feb 26;60(1):85-9. doi: 10.1007/BF00495731.

DOI:10.1007/BF00495731
PMID:429214
Abstract

Alkaline phosphatase activity has been investigated by histochemical methods in normal and diseased human large intestine. The tissues were constantly maintained at 4 degrees C or below. Specimens were either frozen in liquid nitrogen, freeze-dried and embedded in glycol methacrylate for sectioning at 2 mu, or, fixed in ice-cold formol-calcium for frozen sectioning at 10 mu. The simultaneous coupling azo dye method using the substrates sodium alpha-naphthyl phosphate and Naphthol AS-BI phosphate, resulted in the demonstration of alkaline phosphatase activity in the surface epithelial cells, and the middle and upper crypts, of normal and transitional mucosa.

摘要

相似文献

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Histochemical demonstration of alkaline phosphatase in human large intestine, normal and diseased.
Histochemistry. 1979 Feb 26;60(1):85-9. doi: 10.1007/BF00495731.
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本文引用的文献

1
The distribution of certain enzyme systems in the normal human gastrointestinal tract.某些酶系统在正常人体胃肠道中的分布。
Gastroenterology. 1963 Jun;44:745-60.
2
Histochemical comparison of naphthol AS-phosphates for the demonstration of phosphatases.用于磷酸酶显示的萘酚AS-磷酸盐的组织化学比较
J Natl Cancer Inst. 1958 Mar;20(3):601-15.
3
Adenocarcinoma, adenomas and polyps of the colon. Histochemical study.结肠腺癌、腺瘤及息肉。组织化学研究。
丁酸钠诱导小肠上皮细胞系IEC6中肝型碱性磷酸酶活性。
Dig Dis Sci. 1998 May;43(5):1116-23. doi: 10.1023/a:1018807524075.
4
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Dig Dis Sci. 1996 Apr;41(4):727-39. doi: 10.1007/BF02213129.
5
Serum free medium increases expression of markers of differentiation in human colonic crypt cells.无血清培养基可增加人结肠隐窝细胞中分化标志物的表达。
Gut. 1994 Jun;35(6):791-7. doi: 10.1136/gut.35.6.791.
6
Ultrastructural localisation of alkaline phosphatase in adult human large intestine.碱性磷酸酶在成人大肠中的超微结构定位
Gut. 1982 Feb;23(2):134-6. doi: 10.1136/gut.23.2.134.
Cancer. 1968 Feb;21(2):165-77. doi: 10.1002/1097-0142(196802)21:2<165::aid-cncr2820210202>3.0.co;2-e.
4
The significance of enzyme histochemical patterns in carcinomas of the large intestine in man.人结肠癌中酶组织化学模式的意义。
Gut. 1973 Jun;14(6):502-5. doi: 10.1136/gut.14.6.502.
5
Freeze-drying and embedding in glycol methacrylate (GMA). The results of morphological, histochemical and immunohistological investigations.冷冻干燥并包埋于甲基丙烯酸乙二醇酯（GMA）中。形态学、组织化学和免疫组织学研究结果。
Histochemistry. 1974 Jun 5;39(4):312-26. doi: 10.1007/BF00495682.
6
A simple method of preparing 1-2 microm sections of large tissue blocks using glycol methacrylate.一种使用甲基丙烯酸乙二醇酯制备大型组织块1-2微米切片的简单方法。
J Microsc. 1974 Jul;101(Pt 2):223-7. doi: 10.1111/j.1365-2818.1974.tb03889.x.
7
Science and art in preparing tissues embedded in plastic for light microscopy, with special reference to glycol methacrylate, glass knives and simple stains.用于光学显微镜检查的塑料包埋组织制备中的科学与艺术，特别涉及甲基丙烯酸乙二醇酯、玻璃刀和简单染色法。
Stain Technol. 1976 Mar;51(2):71-97. doi: 10.3109/10520297609116677.