多瘤病毒、猴病毒40或腺病毒12转化细胞中存在的叙利亚仓鼠核糖核酸的特征。
Characteristics of the syrian hamster ribonucleic acid present in cells transformed by polyoma, simian virus 40, or adenovirus 12.
作者信息
Martin M A
出版信息
J Virol. 1969 Feb;3(2):119-25. doi: 10.1128/JVI.3.2.119-125.1969.
Abstract
Ribonucleic acids of known specific activities were separately prepared from hamster cells transformed by polyoma, simian virus 40 (SV40), and adenovirus 12. When increasing amounts of these labeled ribonucleic acids were added to nitrocellulose filters containing immobilized (14)C-hamster deoxyribonucleic acid (DNA), about 5% of the DNA sites were saturated in each case. An additive saturation-hybridization experiment indicated that a portion of the 5% of the hamster DNA transcribed in SV40-transformed cells was also transcribed in polyoma-transformed cells. In addition, a separate region of the hamster genome was also transcribed in polyoma-transformed cells.
摘要
分别从经多瘤病毒、猴病毒40（SV40）和腺病毒12转化的仓鼠细胞中制备了具有已知比活性的核糖核酸。当将越来越多的这些标记核糖核酸添加到含有固定化（14）C - 仓鼠脱氧核糖核酸（DNA）的硝酸纤维素滤膜上时，每种情况下约5%的DNA位点被饱和。一项加性饱和杂交实验表明，在SV40转化细胞中被转录的5%仓鼠DNA的一部分，在多瘤病毒转化细胞中也被转录。此外，仓鼠基因组的一个单独区域在多瘤病毒转化细胞中也被转录。
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