在区带超速离心机中对疱疹病毒（单纯疱疹病毒、巨细胞病毒和EB病毒）进行浓缩和纯化。
Concentration and purification of herpesviruses (simplex, cytomegalo, and EB) in a zonal ultracentrifuge.
作者信息
McCombs R M
出版信息
Appl Microbiol. 1969 Apr;17(4):636-8. doi: 10.1128/am.17.4.636-638.1969.
Abstract
By using the BXIV zonal ultracentrifuge rotor, it was possible to efficiently concentrate and partially purify members of the herpesvirus group by pelleting the virus onto a double sucrose cushion.
摘要
通过使用BXIV区带超速离心机转头，能够通过将病毒沉淀到双层蔗糖垫层上，有效地浓缩和部分纯化疱疹病毒组的成员。
相似文献
1
Concentration and purification of herpesviruses (simplex, cytomegalo, and EB) in a zonal ultracentrifuge.在区带超速离心机中对疱疹病毒（单纯疱疹病毒、巨细胞病毒和EB病毒）进行浓缩和纯化。
Appl Microbiol. 1969 Apr;17(4):636-8. doi: 10.1128/am.17.4.636-638.1969.
2
Studies on EB virus of Burkitt's lymphoblasts.伯基特淋巴母细胞的EB病毒研究。
Virology. 1969 Oct;39(2):312-21. doi: 10.1016/0042-6822(69)90051-8.
3
Persistent infections with herpesviruses.疱疹病毒的持续性感染。
Prog Med Virol. 1974;18(0):160-77.
4
Studies of Burkitt lymphoma cells. 3. Equilibrium density gradient centrigugation of virus particles isolated from Burkitt lymphoma cell lines.
Cancer. 1969 Feb;23(2):300-5. doi: 10.1002/1097-0142(196902)23:2<300::aid-cncr2820230206>3.0.co;2-4.
5
Herpesviruses and cancer.疱疹病毒与癌症。
Adv Cancer Res. 1974;19(0):265-302. doi: 10.1016/s0065-230x(08)60056-1.
6
Formal discussion: immunologic relationships of the herpes-like EB virus of cultured Burkitt lymphoblasts.正式讨论：培养的伯基特淋巴母细胞中类疱疹EB病毒的免疫学关系。
Cancer Res. 1967 Dec;27(12):2489-93.
7
Concentration of oncogenic herpesviruses by methyl alcohol precipitation.通过甲醇沉淀法浓缩致癌性疱疹病毒。
Appl Microbiol. 1974 May;27(5):988-90. doi: 10.1128/am.27.5.988-990.1974.
8
High incidence of virus isolation from donor and recipient tissues associated with renal transplantation.肾移植供体和受体组织中病毒分离的高发生率。
Nature. 1978 Mar 23;272(5651):372-3. doi: 10.1038/272372a0.
9
Zonal centrifuge applied to the purification of Herpesvirus in the Lucké frog kidney tumor.区带离心机用于纯化勒克氏蛙肾肿瘤中的疱疹病毒。
Appl Microbiol. 1971 Jan;21(1):132-9. doi: 10.1128/am.21.1.132-139.1971.
10
Rapid quantitative PCR assays for the simultaneous detection of herpes simplex virus, varicella zoster virus, cytomegalovirus, Epstein-Barr virus, and human herpesvirus 6 DNA in blood and other clinical specimens.用于同时检测血液及其他临床标本中单纯疱疹病毒、水痘带状疱疹病毒、巨细胞病毒、爱泼斯坦-巴尔病毒和人类疱疹病毒6型DNA的快速定量聚合酶链反应检测法。
J Med Virol. 2008 Mar;80(3):467-77. doi: 10.1002/jmv.21095.
引用本文的文献
1
Long term stability and infectivity of herpesviruses in water.疱疹病毒在水中的长期稳定性和感染力。
Sci Rep. 2017 Apr 21;7:46559. doi: 10.1038/srep46559.
2
Efficacy of a virion envelope herpes simplex virus vaccine against experimental skin infections in hairless mice.一种病毒粒子包膜单纯疱疹病毒疫苗对无毛小鼠实验性皮肤感染的疗效。
Arch Virol. 1981;68(2):73-80. doi: 10.1007/BF01314437.
3
Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors.成年献血者单纯疱疹病毒抗体滴度与特异性淋巴细胞刺激的相关性
J Clin Microbiol. 1981 Jan;13(1):36-41. doi: 10.1128/jcm.13.1.36-41.1981.
4
Propagation and purification of high-titer human cytomegalovirus.高滴度人巨细胞病毒的增殖与纯化
Appl Microbiol. 1971 Nov;22(5):914-8. doi: 10.1128/am.22.5.914-918.1971.
5
Concentration of herpesviruses.疱疹病毒的浓度
J Virol. 1973 Feb;11(2):325-8. doi: 10.1128/JVI.11.2.325-328.1973.
6
Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.巨细胞病毒免疫球蛋白M抗体的固相酶免疫测定
J Clin Microbiol. 1977 Jun;5(6):629-34. doi: 10.1128/jcm.5.6.629-634.1977.
7
Envelope and nucleocapsid antigens of Cytomegalovirus (CMV).巨细胞病毒（CMV）的包膜抗原和核衣壳抗原。
Med Microbiol Immunol. 1975 Jul 2;161(3):155-62. doi: 10.1007/BF02121005.
本文引用的文献
1
Analytical techniques for cell fractions. V. Characteristics of the B-XIV and B-XV zonal centrifuge rotors.细胞组分的分析技术。V. B-XIV和B-XV区带离心机转子的特性
Anal Biochem. 1967 Nov;21(2):235-52. doi: 10.1016/0003-2697(67)90185-6.
2
Theory of centrifugation: miscellaneous studies.离心理论：杂项研究。
Natl Cancer Inst Monogr. 1966 Jun;21:41-76.
3
4
The use of batch-type zonal ultracentrifuge rotors for the isolation and purification of viruses.
J Gen Virol. 1968 May;2(3):455-9. doi: 10.1099/0022-1317-2-3-455.
5
Correlation between infectivity and physical virus particles in human cytomegalovirus.人巨细胞病毒中感染性与物理病毒颗粒之间的相关性
J Bacteriol. 1966 Nov;92(5):1555-61. doi: 10.1128/jb.92.5.1555-1561.1966.
6
Biophysical studies of vesicular stomatitis virus.水疱性口炎病毒的生物物理学研究。
J Bacteriol. 1966 Feb;91(2):803-12. doi: 10.1128/jb.91.2.803-812.1966.
7
Interactions of human cytomegalovirus with human fibroblasts.人巨细胞病毒与人成纤维细胞的相互作用。
J Bacteriol. 1966 Jan;91(1):213-20. doi: 10.1128/jb.91.1.213-220.1966.
Zotero 插件