Benyesh-Melnick M, Probstmeyer F, McCombs R, Brunschwig J P, Vonka V
J Bacteriol. 1966 Nov;92(5):1555-61. doi: 10.1128/jb.92.5.1555-1561.1966.
Benyesh-Melnick, Matilda (Baylor University College of Medicine, Houston, Tex.), Fern Probstmeyer, Robert McCombs, Jean P. Brunschwig, and Vladimir Vonka. Correlation between infectivity and physical virus particles in human cyto-megalovirus. J. Bacteriol. 92:1555-1561. 1966.-Infectivity titers [measured as plaque-forming units (PFU)] and particle counts by the sedimentation pseudo-replication technique were determined for crude, unpurified, intracellular preparations of two different strains of human cytomegalovirus. Unlike the high particle-infectivity ratio of 10(6) to 10(8) previously reported for these viruses, the number of total particles per PFU ranged from 160 to 490 with strain AD-169 and from 176 to 1,050 for strain C-87. Interpretation of particle-PFU ratios of intracellular cytomegalovirus in terms of particle morphology is not conclusive at this time. The number of enveloped forms found varied between 0 and 34% of the total particles counted. However, the true proportion is probably greater, because envelopes were found to be destroyed by the enzyme treatment used in preparing the specimens for examination in the electron microscope. The number of full particles found ranged between 4 and 31% of the total particles counted. The particle per PFU ratio of extracellular virus was found to be three- to fivefold lower than that of intracellular virus.
贝涅什 - 梅尔尼克、玛蒂尔达（得克萨斯州休斯顿贝勒医学院）、弗恩·普罗布斯特迈尔、罗伯特·麦库姆斯、让·P·布伦施维格和弗拉基米尔·冯卡。人巨细胞病毒感染性与物理病毒颗粒之间的相关性。《细菌学杂志》92:1555 - 1561。1966年。——对两种不同株的人巨细胞病毒的粗制、未纯化的细胞内制剂，通过沉降假复制技术测定了感染性滴度[以空斑形成单位（PFU）衡量]和颗粒计数。与先前报道的这些病毒10(6)至10(8)的高颗粒感染率不同，AD - 169株每PFU的总颗粒数在160至490之间，C - 87株在176至1050之间。目前根据颗粒形态对细胞内巨细胞病毒的颗粒 - PFU比率进行解释尚无定论。所发现的包膜形式数量在计数的总颗粒数的0%至34%之间变化。然而，真实比例可能更高，因为在制备用于电子显微镜检查的标本时，发现包膜会被酶处理破坏。所发现的完整颗粒数量在计数的总颗粒数的4%至31%之间。发现细胞外病毒的每PFU颗粒比率比细胞内病毒低三至五倍。
