Schmitz H, Doerr H W, Kampa D, Vogt A
J Clin Microbiol. 1977 Jun;5(6):629-34. doi: 10.1128/jcm.5.6.629-634.1977.
A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.
本文描述了一种用于检测巨细胞病毒免疫球蛋白M抗体的灵敏的固相酶免疫测定法。酶免疫测定法的结果与间接免疫荧光法所得结果高度相关。事实证明，辣根过氧化物酶是比碱性磷酸酶更灵敏的标记物。通过使用由巨细胞病毒感染细胞经超声破碎的细胞核制备的核抗原，可避免市售巨细胞病毒抗原出现的非特异性反应。
