Vonka V, Benyesh-Melnick M
J Bacteriol. 1966 Jan;91(1):213-20. doi: 10.1128/jb.91.1.213-220.1966.
Vonka, Vladimir (Baylor University College of Medicine, Houston, Tex.), and Matilda Benyesh-Melnick. Interactions of human cytomegalovirus with human fibroblasts. J. Bacteriol. 91:213-220. 1966.-Virus attachment of human cytomegalovirus to human embryo lung fibroblasts was found to be temperature-independent, from 4 to 37 C. Prolonged incubation at 4 C, however, resulted in inactivation of a high proportion of attached virus. Virus penetration seemed to be temperature-dependent, occurring at 37 C but not at 4 C. Detailed studies of the growth curve of the virus were made. Cell-associated virus preceded the appearance of virus in the fluid phase by 2 to 5 days. Complement-fixing antigen could be detected, but only when the cytopathic effect was advanced, and it was demonstrable only in the cell-associated fraction. Under methyl cellulose, decreasing the bicarbonate concentration in the overlay from 0.225 to 0.15% resulted in marked increase in plating efficiency with all strains tested. However, varying the concentration of bicarbonate from 0.3 to 0.15% in fluid medium did not influence the growth of virus.
冯卡，弗拉基米尔（德克萨斯州休斯顿贝勒医学院），以及玛蒂尔达·贝涅什 - 梅尔尼克。人巨细胞病毒与人成纤维细胞的相互作用。《细菌学杂志》91:213 - 220。1966年。——发现人巨细胞病毒在4至37摄氏度下对人胚肺成纤维细胞的病毒附着与温度无关。然而，在4摄氏度下长时间孵育会导致很大比例的附着病毒失活。病毒穿透似乎与温度有关，在37摄氏度时发生而在4摄氏度时不发生。对该病毒的生长曲线进行了详细研究。细胞相关病毒比液相中病毒的出现早2至5天。补体结合抗原可以被检测到，但仅在细胞病变效应进展时，并且仅在细胞相关部分可证实。在甲基纤维素下，将覆盖层中的碳酸氢盐浓度从0.225%降至0.15%会导致所有测试菌株的平板接种效率显著提高。然而，在液体培养基中将碳酸氢盐浓度从0.3%变为0.15%并不影响病毒的生长。
