Bader J P, Steck T L
J Virol. 1969 Oct;4(4):454-9. doi: 10.1128/JVI.4.4.454-459.1969.
Cells producing the Rauscher strain of murine leukemia virus (MLV) were exposed to (3)H-uridine, and labeled virus was collected at hourly intervals. Ribonucleic acid (RNA) extracted from virions (vRNA) had a characteristic single peak when analyzed by electrophoresis in polyacrylamide-agarose composite gels. Exposure of vRNA to dimethyl sulfoxide, urea, formaldehyde, or heat altered the mobility to a faster moving form (vRNA'). This vRNA' sedimented more slowly than native vRNA in sucrose gradients. Incubation of labeled virions at 37 C resulted in fragmentation of viral RNA which was detectable only after denaturation. Also, large differences in the temperature required for the change from vRNA to vRNA' were seen with alterations in NaCl concentration. These experiments demonstrate that the vRNA of MLV is held in a specific conformation by hydrogen bonds distributed over a large part of the molecule. The possibility that an undefined factor is associated with viral RNA is discussed.
将产生劳氏鼠白血病病毒（MLV）的细胞暴露于³H-尿苷，并每隔一小时收集标记的病毒。从病毒粒子中提取的核糖核酸（RNA）（病毒RNA，vRNA）在聚丙烯酰胺-琼脂糖复合凝胶中进行电泳分析时具有特征性的单峰。将vRNA暴露于二甲基亚砜、尿素、甲醛或加热会使其迁移率变为更快移动的形式（vRNA'）。在蔗糖梯度中，这种vRNA'的沉降速度比天然vRNA慢。将标记的病毒粒子在37℃孵育会导致病毒RNA片段化，这只有在变性后才能检测到。此外，随着NaCl浓度的改变，从vRNA转变为vRNA'所需的温度也有很大差异。这些实验表明，MLV的vRNA通过分布在分子大部分区域的氢键保持在特定构象中。文中讨论了与病毒RNA相关的未定义因素的可能性。
