Dunnebacke T H, Levinthal J D, Williams R C
J Virol. 1969 Oct;4(4):505-13. doi: 10.1128/JVI.4.4.505-513.1969.
Cells of differing culture types were inoculated with poliovirus at 37 C, sampled at intervals during the replicative cycle, and examined in thin sections by electron microscopy. The earliest samples, taken at 2 and 5 min postinoculation, showed virus particles adjacent to the exterior of the plasma membrane and others that had apparently penetrated it directly; later samples showed fewer such particles or none. Particles lying in the peripheral cytoplasm frequently appeared swollen and distorted in shape. No sign of virus entry by a pinocytotic process was found at any time. At 3 hr, and subsequently during the replication cycle, particles of progeny virus appeared in the cytoplasm. They were found free in the cytoplasmic matrix, aligned along the elements of filamentous complexes, and enclosed within vesicles. Some of the vesicles were found to be open to the extracellular space, indicating a likely mechanism of virus release.
将不同培养类型的细胞在37℃下接种脊髓灰质炎病毒，在复制周期内定期取样，并通过电子显微镜对薄片进行检查。接种后2分钟和5分钟采集的最早样本显示，病毒颗粒位于质膜外部附近，还有一些显然直接穿透了质膜；后来的样本显示此类颗粒较少或没有。位于周边细胞质中的颗粒形状经常出现肿胀和扭曲。在任何时候都未发现通过胞饮过程进入病毒的迹象。在3小时及随后的复制周期中，子代病毒颗粒出现在细胞质中。它们在细胞质基质中自由存在，沿着丝状复合体的成分排列，并被包裹在囊泡内。发现一些囊泡通向细胞外空间，这表明了一种可能的病毒释放机制。
