Daniels C A, Borsos T, Rapp H J, Snyderman R, Notkins A L
Proc Natl Acad Sci U S A. 1970 Mar;65(3):528-35. doi: 10.1073/pnas.65.3.528.
Herpes simplex virus which had been sensitized with immunoglobulin M antibody was neutralized by serum deficient in the fifth and sixth components of complement (C) but not by serum deficient in the fourth component C (C4). The sequential addition of the functionally purified components of C showed that the activated first component of C (C1[unk]) failed to neutralize sensitized virus. However, in the presence of an optimal concentration of C1[unk], the addition of C4 resulted in neutralization. The amount of virus neutralized was dependent upon the concentration of immunoglobulin M used to sensitize the virus and the concentration of C1[unk] and C4. The addition of the second component of C (C2) to reaction mixtures containing an optimal concentration of C1[unk] and a limiting concentration of C4 resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C2. The addition of the third component of C (C3) to reaction mixtures containing an optimal concentration of C1[unk] and limiting concentrations of C4 and C2 also resulted in increased neutralization and the amount of virus neutralized was dependent upon the concentration of C3.
已被免疫球蛋白M抗体致敏的单纯疱疹病毒，可被缺乏补体（C）第五和第六成分的血清中和，但不能被缺乏第四成分C（C4）的血清中和。依次添加功能纯化的补体成分表明，活化的补体第一成分（C1[unk]）不能中和致敏病毒。然而，在存在最佳浓度的C1[unk]时，添加C4可导致中和作用。被中和的病毒量取决于用于致敏病毒的免疫球蛋白M的浓度以及C1[unk]和C4的浓度。向含有最佳浓度的C1[unk]和有限浓度的C4的反应混合物中添加补体第二成分（C2），可导致中和作用增强，且被中和的病毒量取决于C2的浓度。向含有最佳浓度的C1[unk]以及有限浓度的C4和C2的反应混合物中添加补体第三成分（C3），也可导致中和作用增强，且被中和的病毒量取决于C3的浓度。
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