Hall W T, Hartley J W, Sanford K K
J Virol. 1968 Mar;2(3):238-47. doi: 10.1128/JVI.2.3.238-247.1968.
Four murine tissue culture cell strains, which originated by cloning from one common cell of subcutaneous connective tissue origin, were examined for the presence of virus by electron microscopy and complement fixation techniques. The relative distribution of C particles and intracisternal A particles was determined. Thereafter, the characteristics of and relationship between A- and C-type particles were investigated. Cell extracts were passaged onto virus-free Swiss and C3Hf mouse embryo tissue cultures; CF and EM tests were again made to investigate the infective capacities of the various particle types. Although C particles were found in passage cultures exposed to extracts from the C particle-bearing strains, no A particles were found in any passage culture. These results indicate that the intracisternal A particle was neither infective nor developmentally associated with the C particle. A positive CF test was correlated with the presence of morphologically detectable C particles and was independent of the concentration of A particles.
对4株源自皮下结缔组织的一个共同细胞克隆的小鼠组织培养细胞株，采用电子显微镜和补体结合技术检测病毒的存在情况。测定了C型颗粒和核内A颗粒的相对分布。此后，研究了A型和C型颗粒的特征及它们之间的关系。将细胞提取物接种到无病毒的瑞士小鼠和C3Hf小鼠胚胎组织培养物上；再次进行补体结合试验（CF）和电子显微镜检测（EM），以研究各种颗粒类型的感染能力。虽然在接种了含C型颗粒菌株提取物的传代培养物中发现了C型颗粒，但在任何传代培养物中均未发现A型颗粒。这些结果表明，核内A颗粒既无感染性，也与C型颗粒没有发育上的关联。补体结合试验呈阳性与形态学上可检测到的C型颗粒的存在相关，且与A型颗粒的浓度无关。
