Edelman R, Wheelock E F
J Virol. 1967 Dec;1(6):1139-49. doi: 10.1128/JVI.1.6.1139-1149.1967.
Each major leukocyte type of the peripheral blood of healthy donors was studied in vitro for its ability to support vesicular stomatitis virus (VSV) replication. Purified cultures of each white blood cell type were prepared by the selective adsorption and elution of cells from silicone-treated glass beads. It was found that monocytes and macrophages (derived from the rapid transformation of monocytes in vitro) were the principal host cells for VSV replication. Interferon added to mixed leukocyte cultures, prior to virus inoculation, reduced virus yields and prevented destruction of macrophages. Cultures of small lymphocytes, containing no detectable monocytes or macrophages, produced amounts of virus equivalent to 1% of that produced in leukocyte cultures which contained 7% monocytes. Small lymphocytes did not undergo demonstrable cytopathic alterations in virus-infected cultures. VSV neither replicated nor produced cytopathic effects in polymorphonuclear leukocytes.
对健康供体外周血中每种主要白细胞类型在体外支持水疱性口炎病毒（VSV）复制的能力进行了研究。通过从硅胶处理过的玻璃珠上选择性吸附和洗脱细胞，制备了每种白细胞类型的纯化培养物。结果发现，单核细胞和巨噬细胞（由体外单核细胞快速转化而来）是VSV复制的主要宿主细胞。在接种病毒之前向混合白细胞培养物中添加干扰素，可降低病毒产量并防止巨噬细胞被破坏。不含可检测到的单核细胞或巨噬细胞的小淋巴细胞培养物产生的病毒量相当于含7%单核细胞的白细胞培养物产生量的1%。在病毒感染的培养物中，小淋巴细胞未出现明显的细胞病变改变。VSV在多形核白细胞中既不复制也不产生细胞病变效应。
