感染多瘤病毒的组织培养细胞的行为
Behavior of tissue culture cells infected with polyoma virus.
作者信息
Dulbecco R
出版信息
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1214-20. doi: 10.1073/pnas.67.3.1214.
Abstract
Infection of tissue culture cells with the oncogenic polyoma virus, or its temperature-sensitive mutant Ts-a, causes several changes other than the previously known induction of cellular DNA synthesis. Cellular movement, survival, and mitotic rate are enhanced in low-serum medium, and morphology is changed; the cellular growth parameters, wound serum requirement, and topoinhibition are markedly decreased. The changes are similar to those that occur in cell transformation and are produced by viral functions known to be expressed in transformed cells. Clues as to the possible mechanisms of all these changes are analyzed and a possible mechanism is discussed.
摘要
用致癌性多瘤病毒或其温度敏感突变体Ts-a感染组织培养细胞，除了先前已知的诱导细胞DNA合成外，还会引起其他几种变化。在低血清培养基中，细胞运动、存活和有丝分裂率增强，形态发生改变；细胞生长参数、伤口血清需求和接触抑制明显降低。这些变化与细胞转化中发生的变化相似，是由已知在转化细胞中表达的病毒功能产生的。分析了所有这些变化可能的机制线索，并讨论了一种可能的机制。
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