来自芽孢杆菌噬菌体φ105的脱氧核糖核酸的结构与生物活性：大肠杆菌核酸外切酶的作用
Structure and biological activity of deoxyribonucleic acid from Bacillus bacteriophage phi 105: effects of Escherichia coli exonucleases.
作者信息
Armentrout R W, Skoog L, Rutberg L
出版信息
J Virol. 1971 Mar;7(3):359-71. doi: 10.1128/JVI.7.3.359-371.1971.
The effects of Escherichia coli exonuclease I, exonuclease III, and deoxyribonucleic acid (DNA) polymerase on the biological activity of mature DNA from temperate Bacillus bacteriophage phi105 were investigated. Intact DNA loses infectivity rapidly upon exposure to exonuclease III. Although there is an overall decrease in marker rescue from exonuclease III-digested DNA, digestion preferentially affects markers at the end of the genetic map. This is taken to indicate a nonpermuted gene sequence in mature DNA. Incubation of mature DNA in the presence of exonuclease I or DNA polymerase has no effect on its biological activity. The possible structure of the ends of mature phi105 DNA is discussed. The rate of digestion of mature phi105 DNA by exonuclease III is only about 1/20 the rate of lambda DNA. Results of digestion of various DNA substrates by exonuclease III indicate that the enzyme distinguishes between different DNA terminal structures.
研究了大肠杆菌核酸外切酶I、核酸外切酶III和脱氧核糖核酸（DNA）聚合酶对温和型芽孢杆菌噬菌体phi105成熟DNA生物活性的影响。完整的DNA在暴露于核酸外切酶III后会迅速丧失感染力。虽然从核酸外切酶III消化的DNA中进行标记拯救总体上有所减少，但消化优先影响遗传图谱末端的标记。这被认为表明成熟DNA中的基因序列是未重排的。在核酸外切酶I或DNA聚合酶存在的情况下孵育成熟DNA对其生物活性没有影响。讨论了成熟phi105 DNA末端的可能结构。核酸外切酶III对成熟phi105 DNA的消化速率仅约为λDNA消化速率的1/20。核酸外切酶III对各种DNA底物的消化结果表明，该酶能区分不同的DNA末端结构。
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