Flock J I
Mol Gen Genet. 1977 Oct 24;155(3):241-7. doi: 10.1007/BF00272803.
Six deletion mutants of temperate Bacillus subtilis phage phi105 have been isolated on the basis of their increased resistance to chelating agents. The size and position of the deletions was determined by electronmicroscopy of DNA heteroduplexes. All deletions are located in a region about 55-70% from one end of the DNA molecule, in the right half of the known genetic map of the phage. The segment 55-65% does not contain any genes essential for lytic growth or lysogenization. A gene(s) for immunity is located in a segment 65-70% from the left end. By electronmicroscopy by partially denatured phi105 DNA two A-T rich regions have been localized in the right half of the molecule. One of these regions falls within the non-essential 55-65% DNA segment.
已基于对螯合剂的抗性增强，分离出了6种温和型枯草芽孢杆菌噬菌体phi105的缺失突变体。通过DNA异源双链体的电子显微镜观察确定了缺失的大小和位置。所有缺失均位于DNA分子一端约55 - 70%的区域，即在噬菌体已知遗传图谱的右半部分。55 - 65%的片段不包含任何裂解生长或溶原化所必需的基因。免疫基因位于距左端65 - 70%的片段中。通过对部分变性的phi105 DNA进行电子显微镜观察，在分子的右半部分定位了两个富含A - T的区域。其中一个区域位于非必需的55 - 65% DNA片段内。
