London J, Meyer E Y, Kulczyk S
J Bacteriol. 1971 Apr;106(1):126-37. doi: 10.1128/jb.106.1.126-137.1971.
Representatives of both Streptococcus faecalis and Lactobacillus casei produce isofunctional malic enzymes. All 10 strains of S. faecalis tested could be induced to synthesize malic enzyme and readily adapted to growth on malate. Although 17 of 21 L. casei strains could be induced to produce malic enzyme, only 9 of 14 strains tested grew at the expense of malate. A comparison of catalytic and regulatory properties suggested that the malic enzymes from S. faecalis and L. casei were very similar. Immunological analyses showed that the numerous similarities in function actually reflected partial protein homologies; however, two distinct forms of the malic enzyme were detected among different strains of L. casei by immunochemical and serological procedures. The division of L. casei into two subgroups based on the immunological type of malic enzyme synthesized corresponds to two subspecies currently recognized by microbial taxonomists.
粪肠球菌和干酪乳杆菌的代表菌株都会产生同功苹果酸酶。所检测的全部10株粪肠球菌都能被诱导合成苹果酸酶，并易于适应在苹果酸盐上生长。虽然21株干酪乳杆菌中有17株能被诱导产生苹果酸酶，但所检测的14株菌株中只有9株能利用苹果酸盐生长。对催化特性和调节特性的比较表明，粪肠球菌和干酪乳杆菌的苹果酸酶非常相似。免疫学分析表明，功能上的众多相似性实际上反映了部分蛋白质的同源性；然而，通过免疫化学和血清学方法在不同的干酪乳杆菌菌株中检测到了两种不同形式的苹果酸酶。根据所合成苹果酸酶的免疫类型将干酪乳杆菌分为两个亚群，这与微生物分类学家目前认可的两个亚种相对应。
