Jørgensen J, Nielsen M, Nielsen C B, Nørmark J
Vox Sang. 1979;36(3):186-91. doi: 10.1111/j.1423-0410.1979.tb04422.x.
The sensitivity of the indirect Coombs' test was investigated using saline, albumin, or low ionic strength (LIS) in the incubation phase and an incubation time varying from 5 to 120 min. Titration of 41 antibodies showed that LIS always resulted in a higher or almost the same mean scores as the other solutions after the same incubation time. The maximum mean score was always higher with LIS than with saline and was reached after a shorter period of incubation, only 20--40 min even with the weakest antibodies. With LIS, a longer incubation time than 40 min resulted in a sudden decrease in mean score. The maximum mean score using saline or albumin was reached after 40--60 min incubation. The same mean score was obtained after only 15--20 min, when LIS was used. Albumin gave only slightly higher mean scores than saline.
在孵育阶段使用生理盐水、白蛋白或低离子强度（LIS）以及5至120分钟不等的孵育时间，对间接抗人球蛋白试验的敏感性进行了研究。对41种抗体的滴定显示，在相同孵育时间后，LIS产生的平均得分总是高于或几乎与其他溶液相同。使用LIS时，最大平均得分总是高于生理盐水，并且在较短的孵育期后即可达到，即使是最弱的抗体也只需20至40分钟。使用LIS时，孵育时间超过40分钟会导致平均得分突然下降。使用生理盐水或白蛋白时，孵育40至60分钟后达到最大平均得分。使用LIS时，仅需15至20分钟就能获得相同的平均得分。白蛋白产生的平均得分仅略高于生理盐水。
