Hehlmann R, Kufe D, Spiegelman S
Proc Natl Acad Sci U S A. 1972 Feb;69(2):435-9. doi: 10.1073/pnas.69.2.435.
Molecular hybridization with radioactively labeled DNA complementary to the RNA of the Rauscher leukemia virus was used to probe for homologous RNA in the polysome fraction of human leukemic cells. The leukocytes of 24 out of 27 patients examined contained RNA possessing homology to that of the mouse leukemia agent, but not to that of the unrelated viruses causing mammary tumors in mice or myeloblastosis in chickens. Further, no control human leukocytes or other adult and fetal tissues showed significant levels of the leukemia-specific RNA. It would appear that human leukemic cells contain RNA sequences homologous to those found in a viral agent known to cause leukemia in an experimental animal. The fact that human sarcomas have also been shown to contain this type of RNA points to a remarkable parallelism in the leukemias and sarcomas of mice and men.
利用与劳斯氏白血病病毒RNA互补的放射性标记DNA进行分子杂交，来探测人类白血病细胞多核糖体组分中的同源RNA。在检测的27例患者中，有24例患者的白细胞含有与小鼠白血病因子RNA同源的RNA，但与在小鼠中引发乳腺肿瘤或在鸡中引发成髓细胞血症的无关病毒的RNA不同源。此外，对照人类白细胞或其他成人及胎儿组织均未显示出白血病特异性RNA的显著水平。看来人类白血病细胞含有与已知在实验动物中引发白血病的病毒因子中发现的RNA序列同源的RNA序列。人类肉瘤也已被证明含有这种类型的RNA，这一事实表明小鼠和人类的白血病和肉瘤之间存在显著的平行关系。
