Urquhart G E, Walker G H
J Clin Pathol. 1972 Oct;25(10):843-5. doi: 10.1136/jcp.25.10.843.
A comparison of immunofluorescent tests for the diagnosis of respiratory syncytial (RS) virus infections was carried out on 42 hospitalized cases of respiratory infection in childhood. Respiratory syncytial virus was detected in 22 (52%) cases, the most sensitive method of detection being by indirect immunofluorescence of Bristol HeLa tissue cultures inoculated with nasopharyngeal aspirates. The highest detection rate was in bronchiolitis cases (92%). Detection of antibody rises in paired sera, eight days apart, confirmed RS virus infection in 13 of 16 cases, the most sensitive test being detection of a specific rise in IgG antibody by indirect immunofluorescence. A serodiagnosis was made in all 10 non-bronchiolitis cases. Recommendations are made for the application ofimmunofluorescence to routine diagnosis of RS virus infection.
对42例儿童住院呼吸道感染病例进行了免疫荧光试验，以比较其对呼吸道合胞（RS）病毒感染的诊断价值。在22例（52%）病例中检测到呼吸道合胞病毒，最敏感的检测方法是用接种鼻咽抽吸物的布里斯托尔人宫颈癌细胞系组织培养物进行间接免疫荧光检测。检出率最高的是细支气管炎病例（92%）。对间隔8天的双份血清进行抗体升高检测，在16例病例中的13例中证实了RS病毒感染，最敏感的检测方法是通过间接免疫荧光检测IgG抗体的特异性升高。所有10例非细支气管炎病例均作出了血清学诊断。文中对免疫荧光法在RS病毒感染常规诊断中的应用提出了建议。
