Cradock-Watson J E, McQuillin J, Gardner P S
J Clin Pathol. 1971 May;24(4):308-12. doi: 10.1136/jcp.24.4.308.
The immunofluorescent examination of nasopharyngeal secretions has been compared with conventional cell culture for the diagnosis of respiratory syncytial (RS) virus infection in children under the age of 2 years in Newcastle upon Tyne and Manchester. Two hundred and sixty-eight identifications of RS virus were made by the fluorescent method, of which 258 (96%) were confirmed culturally. In 267 children from whom virus was isolated in cell culture a positive diagnosis was made by the fluorescent method in 258 (97%). In 238 infants with bronchiolitis a diagnosis of RS virus infection was made on the basis of the fluorescent test in 185 (78%) of which 180 (97%) were subsequently confirmed in cell culture. The reasons for false results are briefly discussed. It is concluded that the method is reliable for the early diagnosis of respiratory syncytial virus infection.
在泰恩河畔纽卡斯尔和曼彻斯特，对2岁以下儿童呼吸道合胞（RS）病毒感染的诊断，将鼻咽分泌物的免疫荧光检查与传统细胞培养法进行了比较。通过荧光法对268例RS病毒进行了鉴定，其中258例（96%）经培养得到证实。在267例通过细胞培养分离出病毒的儿童中，荧光法作出阳性诊断的有258例（97%）。在238例患细支气管炎的婴儿中，根据荧光试验诊断为RS病毒感染的有185例（78%），其中180例（97%）随后经细胞培养得到证实。简要讨论了出现假结果的原因。结论是该方法对呼吸道合胞病毒感染的早期诊断是可靠的。
