Lehner T, Wilton J M, Shillitoe E J, Ivanyi L
Br J Cancer. 1973 May;27(5):351-61. doi: 10.1038/bjc.1973.43.
Cell-mediated and humoral immune responses to Herpesvirus hominis type 1 (HVH1) and Candida albicans were studied in patients with leukoplakia, showing a histological spectrum of changes from epithelial keratosis to acanthosis and atypia, and in patients with carcinoma. The results were ranked according to increasing values of stimulation indices of lymphocyte transformation to HVH1, and the corresponding macrophage migration inhibition indices, and complement fixing antibody titres of each patient were correlated. This revealed that most patients with epithelial atypia were clustered to that end of the spectrum which had the highest stimulation and migration indices to HVH1; this relationship was not evident with C. albicans. In patients with keratosis and acanthosis there was a significant lack of correlation between lymphocyte transformation and migration inhibition to both HVH1 and C. albicans. In carcinoma the indices of lymphocyte transformation and migration inhibition to HVH1 and C. albicans were depressed. Furthermore, a significant negative correlation was found between lymphocyte transformation and migration inhibition to HVH1, unlike the positive correlation in control subjects. Complement fixing antibodies to HVH1, HVH2, cytomegalovirus and adenovirus, and fluorescent antibodies to C. albicans failed to show a significant change in titre in any one group of subjects tested. The results suggest a cell-mediated immune defect in leukoplakia, with a dissociation between lymphocyte transformation and macrophage migration inhibition to HVH1 and C. albicans in cases of keratosis or acanthosis. A specific increase in cell-mediated immunity to HVH1 in leukoplakia with epithelial atypia and the sequential changes argue in favour of a possible participation of HVH1 in carcinomatous transformation of some leukoplakias.
对患有白斑病（其组织学变化范围从上皮角化病到棘皮症和异型增生）的患者以及患有癌症的患者，研究了针对1型人疱疹病毒（HVH1）和白色念珠菌的细胞介导免疫反应和体液免疫反应。根据淋巴细胞对HVH1转化的刺激指数、相应的巨噬细胞移动抑制指数的增加值对结果进行排序，并将每位患者的补体结合抗体滴度进行关联分析。结果显示，大多数上皮异型增生患者聚集在对HVH1具有最高刺激和移动指数的频谱末端；而对于白色念珠菌，这种关系并不明显。在角化病和棘皮症患者中，针对HVH1和白色念珠菌的淋巴细胞转化与移动抑制之间明显缺乏相关性。在癌症患者中，针对HVH1和白色念珠菌的淋巴细胞转化和移动抑制指数均降低。此外，与对照组的正相关不同，发现针对HVH1的淋巴细胞转化与移动抑制之间存在显著的负相关。针对HVH1、HVH2、巨细胞病毒和腺病毒的补体结合抗体，以及针对白色念珠菌的荧光抗体，在任何一组受试对象中均未显示滴度有显著变化。结果表明白斑病存在细胞介导的免疫缺陷，在角化病或棘皮症病例中，针对HVH1和白色念珠菌的淋巴细胞转化与巨噬细胞移动抑制之间存在分离。在伴有上皮异型增生的白斑病中，针对HVH1的细胞介导免疫特异性增加，且存在一系列变化，这表明HVH1可能参与了某些白斑病的癌变转化。
