通过在固相免疫吸附剂上进行亲和层析法纯化小鼠干扰素。
Purification of mouse interferon by affinity chromatography on a solid-phase immunoadsorbent.
作者信息
Sipe J D, de Maeyer-Guignard J, Fauconnier B, de Maeyer E
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1037-40. doi: 10.1073/pnas.70.4.1037.
Abstract
A solid-phase immunoadsorbent capable of binding mouse interferon has been prepared. Starting from crude tissue-culture material, interferon could be purified 1990 times in a single step of affinity chromatography. Overall recovery ranged from 55 to 103% with tissue culture and mouse-brain interferon; however, only 5% was obtained with Sendai virus-induced interferon from mouse serum.
摘要
已制备出一种能够结合小鼠干扰素的固相免疫吸附剂。从粗制的组织培养材料开始，干扰素在一步亲和层析中可被纯化1990倍。组织培养干扰素和小鼠脑干扰素的总回收率在55%至103%之间；然而，从小鼠血清中仙台病毒诱导的干扰素仅获得了5%的回收率。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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Chemical coupling of proteins to agarose.蛋白质与琼脂糖的化学偶联。
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Antitumor effects of interferon preparations in mice.干扰素制剂对小鼠的抗肿瘤作用。
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Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
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Affinity chromatography.亲和层析
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J Gen Virol. 1972 May;15(2):129-38. doi: 10.1099/0022-1317-15-2-129.
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Studies on virus-induced interferons produced by the human amniotic membrane and white blood cells.关于人羊膜和白细胞产生的病毒诱导干扰素的研究。
Arch Gesamte Virusforsch. 1971;34(3):232-43. doi: 10.1007/BF01242997.
8
Effect of antilymphocytic serum on circulating interferon in mice as a function of the inducer.抗淋巴细胞血清对小鼠循环干扰素的影响与诱导剂的关系。
Nat New Biol. 1971 Feb 17;229(7):212-4. doi: 10.1038/newbio229212a0.
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