Genigeorgis C, Sakaguchi G, Riemann H
Appl Microbiol. 1973 Jul;26(1):111-5. doi: 10.1128/am.26.1.111-115.1973.
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sensitive test, followed by microslide diffusion, single gel diffusion and electroimmunodiffusion, guinea pig skin test, mouse test, and rabbit ileal loop test.
A型产气荚膜梭菌NCTC 8798芽孢形成培养物产生的肠毒素被纯化至每毫克氮3500个小鼠平均致死剂量的水平。从注射了肠毒素的兔子身上获得了高效价血清，并用于比较产气荚膜梭菌肠毒素血清学检测和生物测定的敏感性。反向被动血凝试验是迄今为止最敏感的检测方法，其次是微量玻片扩散法、单凝胶扩散法和电免疫扩散法、豚鼠皮肤试验、小鼠试验和兔回肠袢试验。
