A型产气荚膜梭菌肠毒素的检测方法。
Assay methods for Clostridium perfringens type A enterotoxin.
作者信息
Genigeorgis C, Sakaguchi G, Riemann H
出版信息
Appl Microbiol. 1973 Jul;26(1):111-5. doi: 10.1128/am.26.1.111-115.1973.
Abstract
Enterotoxin produced by a sporulating culture of Clostridium perfringens type A NCTC 8798 was purified to a level of 3,500 mouse mean lethal doses per mg of nitrogen. High-titer sera were obtained from rabbits injected with enterotoxin and used to compare the sensitivity of serological tests and bioassays for C. perfringens enterotoxin. Reversed passive hemagglutination was by far the most sensitive test, followed by microslide diffusion, single gel diffusion and electroimmunodiffusion, guinea pig skin test, mouse test, and rabbit ileal loop test.
摘要
A型产气荚膜梭菌NCTC 8798芽孢形成培养物产生的肠毒素被纯化至每毫克氮3500个小鼠平均致死剂量的水平。从注射了肠毒素的兔子身上获得了高效价血清，并用于比较产气荚膜梭菌肠毒素血清学检测和生物测定的敏感性。反向被动血凝试验是迄今为止最敏感的检测方法，其次是微量玻片扩散法、单凝胶扩散法和电免疫扩散法、豚鼠皮肤试验、小鼠试验和兔回肠袢试验。
相似文献
1
Assay methods for Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素的检测方法。
Appl Microbiol. 1973 Jul;26(1):111-5. doi: 10.1128/am.26.1.111-115.1973.
2
Antigenic homogeneity of enterotoxin from different agglutinating serotypes of Clostridium perfringens.产气荚膜梭菌不同凝集血清型肠毒素的抗原同质性
Can J Microbiol. 1973 Apr;19(4):521-4. doi: 10.1139/m73-083.
3
In vitro production of Clostridium perfringens enterotoxin and its detection by reversed passive hemagglutination.产气荚膜梭菌肠毒素的体外生产及其反向被动血凝检测
Appl Microbiol. 1973 Sep;26(3):381-5. doi: 10.1128/am.26.3.381-385.1973.
4
Biological characteristics of Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素的生物学特性
Infect Immun. 1971 Aug;4(2):89-96. doi: 10.1128/iai.4.2.89-96.1971.
5
Time of enterotoxin formation and release during sporulation of Clostridium perfringens type A.A型产气荚膜梭菌芽孢形成过程中肠毒素的形成和释放时间。
J Bacteriol. 1973 Feb;113(2):932-6. doi: 10.1128/jb.113.2.932-936.1973.
6
Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.用电免疫扩散法定量产气荚膜梭菌A型肠毒素
Appl Microbiol. 1972 Nov;24(5):801-4. doi: 10.1128/am.24.5.801-804.1972.
7
Simplified method for purification of Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素纯化的简化方法
Appl Microbiol. 1973 Nov;26(5):762-7. doi: 10.1128/am.26.5.762-767.1973.
8
The role of enterotoxin in Clostridium perfringens type A enteritis.A型产气荚膜梭菌肠炎中肠毒素的作用。
Can J Microbiol. 1971 Jul;17(7):987-91. doi: 10.1139/m71-156.
9
Scaled-up production and purification of Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素的扩大生产与纯化
J Appl Bacteriol. 1986 Jun;60(6):517-25. doi: 10.1111/j.1365-2672.1986.tb01091.x.
10
An enterotoxin produced by Clostridium perfringens type D. Purification by affinity chromatography.D型产气荚膜梭菌产生的一种肠毒素。通过亲和层析法纯化。
Acta Pathol Microbiol Scand B. 1976 Dec;84B(6):414-20. doi: 10.1111/j.1699-0463.1976.tb01960.x.
引用本文的文献
1
Diagnostic Accuracy of Nucleic Acid Amplification-Based Assays for Clostridium perfringens-Associated Diseases: a Systematic Review and Meta-analysis.基于核酸扩增检测方法对产气荚膜梭菌相关疾病的诊断准确性：一项系统评价和荟萃分析
J Clin Microbiol. 2020 Aug 24;58(9). doi: 10.1128/JCM.00363-20.
2
Highly sensitive assay for Clostridium perfringens enterotoxin that uses inhibition of plating efficiency of Vero cells grown in culture.用于检测产气荚膜梭菌肠毒素的高灵敏度检测方法，该方法利用抑制培养的Vero细胞的平板接种效率。
J Clin Microbiol. 1981 May;13(5):940-6. doi: 10.1128/jcm.13.5.940-946.1981.
3
Response of ligated intestinal loops in chickens to the enterotoxin of Clostridium perfringens.鸡结扎肠袢对产气荚膜梭菌肠毒素的反应
Appl Microbiol. 1974 Nov;28(5):889-91. doi: 10.1128/am.28.5.889-891.1974.
4
In vitro production of Clostridium perfringens enterotoxin and its detection by reversed passive hemagglutination.产气荚膜梭菌肠毒素的体外生产及其反向被动血凝检测
Appl Microbiol. 1973 Sep;26(3):381-5. doi: 10.1128/am.26.3.381-385.1973.
5
Clinical and antibody responses to Clostridium perfringens type A enterotoxin in experimental sheep and calves.实验性绵羊和犊牛对A型产气荚膜梭菌肠毒素的临床及抗体反应
Can J Comp Med. 1985 Apr;49(2):145-8.
6
Purification by high performance liquid chromatography of Clostridium perfringens type A enterotoxin prepared from high toxin producers selected by a toxin-antitoxin halo.通过高效液相色谱法对由毒素-抗毒素晕圈筛选出的高毒素产生菌株制备的A型产气荚膜梭菌肠毒素进行纯化。
Eur J Epidemiol. 1985 Jun;1(2):131-8. doi: 10.1007/BF00141806.
7
Development and application of an enzyme linked immunosorbent assay for Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素酶联免疫吸附测定法的开发与应用
J Clin Pathol. 1985 Feb;38(2):222-8. doi: 10.1136/jcp.38.2.222.
8
Morphological alterations and changes in cellular cations induced by Clostridium perfringens type A enterotoxin in tissue culture cells.A型产气荚膜梭菌肠毒素在组织培养细胞中诱导的形态学改变及细胞阳离子变化。
Eur J Epidemiol. 1985 Dec;1(4):264-73. doi: 10.1007/BF00237101.
9
Measurement of biological activities of purified and crude enterotoxin of Clostridium perfringens.产气荚膜梭菌纯化及粗制肠毒素生物活性的测定。
Infect Immun. 1975 Aug;12(2):440-2. doi: 10.1128/iai.12.2.440-442.1975.
本文引用的文献
1
Clostridium perfringens Type A Food Poisoning II. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Human Beings.产气荚膜梭菌 A 型食物中毒 II. 兔回肠的反应作为指示人体产气荚膜梭菌菌株肠致病性的指标。
Infect Immun. 1971 Jan;3(1):171-8. doi: 10.1128/iai.3.1.171-178.1971.
2
Clostridium perfringens Type A Food Poisoning I. Response of the Rabbit Ileum as an Indication of Enteropathogenicity of Strains of Clostridium perfringens in Monkeys.产气荚膜梭菌 A 型食物中毒。一、以兔子回肠反应作为指示猴源产气荚膜梭菌菌株肠致病性的依据。
Infect Immun. 1971 Jan;3(1):167-70. doi: 10.1128/iai.3.1.167-170.1971.
3
Mechanism of Action of the Enteropathogenic Factor of Clostridium perfringens Type A.A 型产气荚膜梭菌肠致病因子作用机制的研究
Infect Immun. 1971 Jan;3(1):100-6. doi: 10.1128/iai.3.1.100-106.1971.
4
5
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
6
7
The micro-slide gel double diffusion test for the detection and assay of staphylococcal enterotoxins.用于检测和测定葡萄球菌肠毒素的微量玻片凝胶双向扩散试验。
Health Lab Sci. 1969 Oct;6(4):185-98.
8
Rapid quantitative serological assay of staphylococcal enterotoxin B.葡萄球菌肠毒素B的快速定量血清学检测
Appl Microbiol. 1966 Mar;14(2):284-91. doi: 10.1128/am.14.2.284-291.1966.
9
Quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion.用电免疫扩散法定量产气荚膜梭菌A型肠毒素
Appl Microbiol. 1972 Nov;24(5):801-4. doi: 10.1128/am.24.5.801-804.1972.
10
Purification and biochemical properties of Clostridium perfringens type A enterotoxin.A型产气荚膜梭菌肠毒素的纯化及生化特性
Infect Immun. 1972 Nov;6(5):662-73. doi: 10.1128/iai.6.5.662-673.1972.
Zotero 插件