产气荚膜梭菌纯化及粗制肠毒素生物活性的测定。
Measurement of biological activities of purified and crude enterotoxin of Clostridium perfringens.
作者信息
Nilo L
出版信息
Infect Immun. 1975 Aug;12(2):440-2. doi: 10.1128/iai.12.2.440-442.1975.
Abstract
Enterotoxin of Clostridium perfringens was assayed and compared with toxicity in mice and erythemal activity in guinea pigs. Conversion factors were used to express these biological activities of crude enterotoxin in terms of weight of pure enterotoxin protein. One microgram of enterotoxin was equivalent to 3.41 erythema units and to 0.68 mouse median lethal dose.
摘要
对产气荚膜梭菌肠毒素进行了测定，并在小鼠体内的毒性和豚鼠的红斑活性方面进行了比较。使用换算因子，根据纯肠毒素蛋白的重量来表示粗制肠毒素的这些生物学活性。一微克肠毒素相当于3.41个红斑单位和0.68个小鼠半数致死剂量。
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