Perini A, Mota I
Immunology. 1973 Aug;25(2):297-305.
The adjuvant action of lipopolysaccharide (LPS) on the IgE and IgG1 antibody production by guinea-pigs was studied. It was observed that LPS induces an early preferential production of IgE antibody. The optimal dose of LPS to cause this effect was of the order of 20 μg/kg. Low amounts of LPS as well as low amounts of antigen favoured the production of IgE antibody whereas high amounts of LPS or antigen favoured the production of IgG1. Later, in the course of immunization, the IgE antibody content decreased and the IgG1 antibody content increased. Booster injection of antigen resulted in a considerable and simultaneous increase in both IgE and IgG1. The preferential production of IgE was consistently obtained when LPS and antigen were injected simultaneously. Injection of LPS 24 hours after antigen resulted in a higher production of IgE and IgG1 but in the loss of the preferential production of IgE. Guinea-pig IgE antibody was able to persist in homologous skin for at least 46 days.
研究了脂多糖（LPS）对豚鼠IgE和IgG1抗体产生的佐剂作用。观察到LPS诱导IgE抗体的早期优先产生。引起这种效应的LPS最佳剂量约为20μg/kg。低剂量的LPS以及低剂量的抗原有利于IgE抗体的产生，而高剂量的LPS或抗原则有利于IgG1的产生。后来，在免疫过程中，IgE抗体含量下降，IgG1抗体含量增加。抗原加强注射导致IgE和IgG1同时显著增加。当LPS和抗原同时注射时，始终能优先产生IgE。抗原注射24小时后注射LPS导致IgE和IgG1产生增加，但失去了IgE的优先产生。豚鼠IgE抗体能够在同源皮肤中持续至少46天。
