用31P核磁共振法测量酵母孢子的内部pH值。
Measurement of the internal pH of yeast spores by 31P nuclear magnetic resonance.
作者信息
Barton J K, den Hollander J A, Lee T M, MacLaughlin A, Shulman R G
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2470-3. doi: 10.1073/pnas.77.5.2470.
The asci and single spores of the yeast Pichia pastoris were examined by using 31P NMR. The pH within the spores was determined from the pH-dependent chemical shifts of the internal orthophosphate (Pi) peaks. In spectra of asci, two internal Pi peaks corresponding to two compartments were observed. Only small variations in the internal pH values were found upon incubation of the asci in buffers ranging in pH from 3 to 10. For this range in external pH, the internal pH values calculated from the Pi chemical shifts were 5.5-6.3 and 5.1-5.9. The two internal Pi peaks, which have line widths of approximately 60 Hz, have been assigned to spore and epiplasmic compartments, respectively. Spectra of single spores revealed only one Pi peak, and, upon germination of either the single spores or asci, only this Pi peak disappeared. Peaks corresponding to ATP were not found in the dormant spores and asci but did appear upon germination. These NMR studies show the yeast spore to have a mobile and relatively acidic interior.
利用³¹P核磁共振技术对巴斯德毕赤酵母的子囊和单孢子进行了检测。根据内部正磷酸盐（Pi）峰的pH依赖性化学位移确定孢子内的pH值。在子囊的光谱中，观察到对应于两个区室的两个内部Pi峰。将子囊在pH值范围为3至10的缓冲液中孵育时，发现内部pH值仅有微小变化。对于此外部pH值范围，根据Pi化学位移计算出的内部pH值为5.5 - 6.3和5.1 - 5.9。这两个线宽约为60 Hz的内部Pi峰分别对应于孢子和周质区室。单孢子的光谱仅显示一个Pi峰，并且在单孢子或子囊萌发时，只有这个Pi峰消失。在休眠孢子和子囊中未发现对应于ATP的峰，但在萌发时确实出现了。这些核磁共振研究表明酵母孢子具有可移动且相对酸性的内部环境。
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